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| 响应面法优化蝉棒束孢SLGY-2产孢条件 |
| Optimization sporulation conditionfor Cordyceps cicadae SLGY2 by response surface method |
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| DOI： |
| 中文关键词: 斜纹夜蛾 棒束孢 培养基优化 产孢量 响应面 |
| 英文关键词:Spodoptera litura Cordyceps optimization of medium spore yield response surface |
| 基金项目:中国烟草总公司贵州省公司重大科技专项“贵州烟草主要病虫害绿色防控技术的研究与应用”（201603；201752010040001）；中国烟草总公司贵州省公司科技项目；黔烟科合[2017]12号；贵州省高层次创新型人才（“百”层次）培养项目（黔科合人才[2016]4022号） |
| Author Name | Affiliation | | ZHANG Sheng-Lan，ZOU Xiao，SHANG Sheng-Hua，YU Xiao-Fei，YANG Mao-Fa | 1. Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang 550025, China
2. Institute of Fungus Resource, College of Life Science, Guizhou University, Guiyang 550025, China
3. Guizhou Academy of Tobacco Sciences, Guiyang 550081, China
4. College of Tobacco Science, Guizhou University, Guiyang 550025, China |
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| 中文摘要: |
| 棒束孢菌Cordyceps sp是一类重要的昆虫病原真菌，具有开发潜能。前期（通过生物毒力测定）筛选一株对斜纹夜蛾具有较高毒力蝉棒束孢SLGY-2菌株，为进一步提高其产孢活力，分别利用单因素方差法和中心组合设计配合响应面分析两种方法开展了本次研究。本研究以产孢量为指标，选择培养基营养成分含量、pH值、温度以及光照条件等进行单因素试验，通过响应曲面法优化最佳产孢条件。结果表明：SLGY-2菌株在实际和模拟较为吻合，模型中最佳产孢条件为培养基营养成分含量为38.3 g、pH 6.55、温度25.21℃、光照18.04 h。在该培养条件下，产孢量达到5.70×10^8个/mL,可用于孢子批量生产。 |
| 英文摘要: |
| As an important entomogenous fungus, the Cordyceps sp. has the potential to implement Cordyceps cicadae SLGY2 with high virulence on Spodoptera liturawas acquired from previous work. Two study: 1) one way variance method and 2) central combination design and response surface analysis, were carried out to improve spore yield. Factors included nutrient content, pH value, temperature and illumination conditions of the medium. The optimal sporulation conditions were generated by response surface method with spore yield as the index. Results showed that SLGY2 was correlated with model. In the model, the optimal sporulation conditions were: Nutrient content of the medium was 38.3 g, pH 6.55, 25~21℃ and 18.04 h light. This can be implemented in future study in biocontrol industry. |
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