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| 稻螟赤眼蜂诱导滞育条件的优化 |
| Optimization of the diapause induction condition on Trichogramma japonicum |
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| DOI： |
| 中文关键词: 赤眼蜂 滞育 光周期 诱导始期 诱导历期 |
| 英文关键词:Trichogramma diapause photoperiod inducing initial stage inducing period |
| 基金项目:国家重点研发计划（2016YFD0200800）；现代农业产业技术体系（CARS-01-17）；浙江省农科院2019年度青年人才培养项目 |
| Author Name | Affiliation | | SHI Ya-Dong, TIAN Jun-Ce, WANG Guo-Rong, LU Yan-Hui, ZHENG Xu-Song, LV Zhong-Xian | 1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang Province, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
3. Xiaoshan Rural Agriculture Bureau, Hangzhou 311203, China |
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| 中文摘要: |
| 应用滞育技术是延长赤眼蜂货架期的可行性措施之一。为实现工厂化生产稻螟赤眼蜂滞育蜂，本研究以在3种光周期（16 L∶8 D、12 L∶12 D和8 L∶16 D）下繁育的稻螟赤眼蜂种群为材料，考察了诱导始期（寄生后发育12 h、24 h、36 h、48 h、60 h和72 h）、诱导温度（10℃和13℃）和诱导历期（14 d、21 d、28 d和35 d）对稻螟赤眼蜂滞育的影响。研究结果表明短光照下繁育的种群更容易进入滞育状态；寄生后发育24 h和12 h为最敏感的诱导始期，随着诱导始期虫龄的增长，滞育效果也随之下降；诱导温度对滞育也有显著影响，其中8 L∶16 D的种群在13℃下诱导的滞育率要显著高于10℃，但其他两个种群在10℃下诱导的滞育率要显著高于13℃；诱导历期的增加可以显著提高稻螟赤眼蜂的滞育率。供试稻螟赤眼蜂种群的较适宜滞育条件为8 L∶16 D下繁育的赤眼蜂种群寄生后发育12 h于13℃下诱导滞育28 d或35 d，以及寄生后发育24 h于10℃或13℃下诱导滞育35 d，滞育率可超过95%。 |
| 英文摘要: |
| Application of diapause technology is one of the feasible measures to extend the shelf life of Trichogramma. In order to realize industrialization of Trichogramma japonicum, three T. japonicumpopulations, which were reared under three different photoperiod (16L∶8D, 12 L∶12 D and 8 L∶16 D), were used in the present study. The effect of inducing initial stage (12 h, 24 h, 36 h, 48 h, 60 and 72 h), inducing temperature (10℃ and 13℃) and inducing period (14 d, 21 d, 28 d and 35 d) on diapause rate of T. japonicumwere investigated.The results showed that the diapause rate of T. japonicum population that reared under photoperiod 8 L∶16 D was higher than the others. 24 h and 12 h are the most sensitive inducing initial stage, and the diapause rate was decreased with the increasing the age of T. japonicum at inducing initial stage. The inducing temperature also had a significant effect on diapause rate. The diapause rate of the 8 L∶16 D population induced at 13℃ was significantly higher than those induced at 10℃. However, the diapause rate of the other two populations induced at 10℃ was significantly higher than 13℃. And the increase of inducing period could significantly improved the diapause rate ofT. japonicum. The optimal conditions for diapause induction of the tested T. japonicum populations were 12 hold of the 8 L∶16 D population induced at 13℃ after 28 d or 35 d, and 24 hold of the 8 L∶16 D population induced at 10 ℃ or 13 ℃ after 35 d. The diapause rate of T. japonicum were above 95% under these induced conditions. |
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