|
| 快速鉴定新菠萝灰粉蚧的分子标记技术 |
| Molecular marker technique for rapid identification of Dysmicoccus neobrevipes (Beardsley) |
| |
| DOI： |
| 中文关键词: 新菠萝灰粉蚧 分子标记 快速鉴定 特异性扩增 |
| 英文关键词:Dysmicoccus neobrevipes (Beardsley) molecular marker rapid identification specific amplification |
| 基金项目:公益性行业（农业）科研专项经费“南繁区生物安全监测预警及控制关键技术研究与示范”（201403075）；国家重点研发计划项目（2016YFC1201200） |
|
| Hits: 928 |
| Download times: 1106 |
| 中文摘要: |
| 新菠萝灰粉蚧Dysmicoccus neobrevipes（Beardsley）是一种严重为害剑麻、番荔枝等经济作物的入侵性害虫。由于粉蚧类昆虫体型较小，且外部形态十分相似，难以达到快速准确识别。本文利用分子标记技术，研究了新菠萝灰粉蚧的快速鉴定方法。通过对新菠萝灰粉蚧、扶桑绵粉蚧、双条拂粉蚧、杰克贝尔氏粉蚧、大洋臀纹粉蚧、木槿曼粉蚧、甘蔗粉蚧和木瓜粉蚧等8种粉蚧的序列进行分析，针对新菠萝灰粉蚧设计了1对特异性引物，其扩增片段大小为552 bp（GenBank登录号为MF 363108），该引物对其它7种粉蚧没有扩增能力。该引物不仅对新菠萝灰粉蚧成虫具有扩增效能，对1龄若虫、2龄若虫和3龄若虫都具有同样的扩增能力，其最低检出阈值为437.5 pg/μL。该技术的建立，实现了在剑麻等苗木调运过程中对新菠萝灰粉蚧的快速准确鉴定，同时对新菠萝灰粉蚧的检疫和监测也具有重要的意义。 |
| 英文摘要: |
| Dysmicoccus neobrevipes(Beardsley) is an invasive pest, which tremendously harms economic crops including Agave sisalana and Annona squamosa. It is very hard to rapidly and accurately distinguishing mealybugs because of their small size and similarity in external morphology. In this study, we had developed a method for the rapid differentiation of D.neobrevipesby molecular marker technique. Sequences of D.neobrevipes、Phenacoccus solenopsis Tinsley、Ferrisia virgata Cockerell、Pseudococcus jackbeardsleyi、Planococcus minor Maskell、Maconellicoccus hirsutus、Saccharicoccus sacchari Cockerell and Paracoccus marginatus wereanalysed. A pair of specific primers which is specific to D.neobrevipes was designed. A single 552 bp (GenBank accession number: MF 363108) fragment was amplified using the specific primers only in D.neobrevipes. No fragments were amplified using the specific primers in other mealybugs. The amplification ability of specific primer was proved to be capable for not only adults but also 1st, 2nd and 3rd instar of D.neobrevipes. Moreover, the assays was capable of detecting 437.5 pg/μL. This technique should be useful tool for pest rapid diagnosis, quarantine and monitoring during transportation of seedlings like A.sisalana. |
| View Full Text View/Add Comment Download reader |
| Close |
About Journal