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| 不同处理菜豆植株对西花蓟马体内解毒酶活性的影响 |
| Effect of different treatments of kidney bean on the activities of detoxification enzymes in Frankliniella occidentalis |
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| DOI： |
| 中文关键词: 西花蓟马 菜豆 诱导抗性 解毒酶 |
| 英文关键词:Frankliniella occidentalis kidney bean induced resistance detoxifying enzymes |
| 基金项目:国家自然科学基金(31660516)；贵州省农业攻关项目（黔科合NY[2015]3014-1号）；贵州省国际科技合作基地（黔科合平台人才[2016]5802） |
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| 中文摘要: |
| 为明确植物的诱导抗性对西花蓟马体内酶活性的影响，通过西花蓟马取食、机械损伤、外源茉莉酸和水杨酸甲酯诱导处理菜豆植株，研究西花蓟马2龄若虫取食不同处理菜豆植株后，其体内多功能氧化酶（MFO）、羧酸酯酶（CarE）、谷胱甘肽S-转移酶（GSTs）、乙酰胆碱酯酶（TChE）的变化。结果表明，西花蓟马2龄若虫取食不同处理的菜豆后，体内多功能氧化酶和乙酰胆碱酯酶活性变化相同，均是在24 h和36 h显著低于取食健康植株的。除取食茉莉酸处理的西花蓟马体内谷胱甘肽S-转移酶活性在24 h受到显著抑制外，西花蓟马若虫取食水杨酸甲酯、蓟马危害后体内的谷胱甘肽S-转移酶活性均高于对照，取食机械损伤处理的西花蓟马体内酶活性在不同取食时间下均与对照差异不显著。取食蓟马危害和茉莉酸处理的西花蓟马虫体羧酸酯酶活性在6 h和36 h均受到显著抑制，而取食机械损伤和水杨酸甲酯诱导的西花蓟马体内酶活性只有在36 h受到明显抑制。以上结果说明外源因子诱导的植物抗性能够干扰西花蓟马体内解毒机制，削弱了其对有毒次生物质的解毒代谢功能。 |
| 英文摘要: |
| To assess the influence of induced resistance of plants on the physiological and biochemical substances in Frankliniella occidentalis. The kidney beans were treated by thrips damage, mechanical damage, exogenous jasmonic acid (JA)and methyl salicylate (MeSA)induction respectively. The changes of activities of multi-function oxidase (MFO), carboxylesterase (CarE), glutathione S-transferase (GSTs), cholinesterase (TChE)in 2ndlarvae ofF.occidentalis feeding different induced kidney bean were evaluated. The results showed that the activities of MFO and TChE in 2nd larvae had similar results, they were significantly lower than that of feeding healthy bean at 24 h and 36 h after F.occidentalis feeding kidney bean leaves of different treatments. The GSTs activity in F.occidentalis increased only at 24 h after thrips feeding kidney bean induced by JA, and the activities of GSTs induced by thrips damage, exogenous MeSA induction were higher than control respectively. GSTs activity in F.occidentalis feeding mechanical damaged plants wasn't significantly different compared with the control under the different feeding time. The CarE activity was significantly inhibited at 6 h and 36 h after feeding the kidney bean plant by thrips damage and JA induced, while it was significantly lower only at 36 h than the control at mechanical damage and MeSA induction. The above results indicated that the induced resistance by exogenous factors interfered with F.occidentalis detoxification mechanisms in the body and reduced its detoxification metabolic function of toxic secondary substance. |
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