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| 移虫日龄对蜂王生长发育的影响 |
| Study on the effect of instar of the grafted larvae on the queen growth and development |
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| DOI： |
| 中文关键词: 蜂王 移虫日龄 生长发育 |
| 英文关键词:Queen bee grafting instar of larvae development |
| 基金项目:国家自然科学基金（31502020，31172272）；现代农业产业技术体系(CARS-45-SYZ6 |
| Author Name | Affiliation | | PANG Qian, SHEN Fang, YIN Ling, JI Ting* | 1.College of Animal Science and Technology, Yangzhou University, Yangzhou 22500, Jiangsu Province, China
2.Fuyang Animal Husbandry and Veterinary Burean,Fuyang 23600,Anhui Province, China
3.Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, Jiangsu Province, China |
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| 中文摘要: |
| 移取1、2、3日龄工蜂幼虫进行人工育王，通过HPLC-MS的方法，分别测定4日龄蜂王幼虫体内保幼激素（JHⅢ）和9日龄蛹体内蜕皮激素（Ecd）的滴度，并通过形态学和解剖学的方法对不同移虫日龄培育的处女王的初生重、卵巢重、头重和卵巢管数进行比较分析，以研究不同移虫日龄对意大利蜂蜂王生长发育的影响。结果显示，不同移虫日龄所培育的蜂王幼虫4日龄JHⅢ和9日龄Ecd浓度均存在显著差异，且随着移虫日龄的增加，蜂王幼虫体内JHⅢ和Ecd含量均显著下降；不同移虫日龄所培育的处女王其初生重、卵巢重、头重及卵巢管数均存在显著差异，且随着移虫日龄的增加，蜂王的初生重、卵巢重和头重显著减轻,蜂王的卵巢管数显著减小；蜂王的初生重和卵巢管数成显著正相关关系，相关系数r=0.82。说明移虫日龄越小幼虫获得的生长条件越好，越有利于蜂王个体尤其是繁殖性状的发育，人工育王实践中应移取虫龄尽量小的幼虫。 |
| 英文摘要: |
| The objective of this study is to research the effect of the instars of the grafted larvae on queen growth and development. The HPLC-MS was used to determine the JHⅢ titer of 4 d larvae and the Ecd titer of 9 d larvae, methods of morphology and anatomy were used to measured correlated parameters on queen of different instar of the grafted larvae. Including the birth weight, ovary weight, head weight and ovariole number. The results indicated that there were significant differences among the JHⅢ titer of 4 days larvae and the Ecd titer of 9 d larvae grafted in different instars. And the older the larvae grafted, the more significant differences showed. And birth weight, ovary weight, head weight and ovariole number were significantly reduced with the increase of grafted instar. The ovaries number is significantly and positively correlate with the birth weight (r=0.82). All of the results indicated that the earlier the larvae grafted, the earlier the better conditions the larvae get, the better development status of the queen. So, we should choose as early as possible of the worker larvae to foster the queen bee. |
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