|
| 利用PiggyBac系统在家蝇中表达蜜蜂王浆蛋白MRJP1 |
| Expression of honey bee royal jelly protein MRJP1 in housefly Musca domestica with a transposon PiggyBac |
| |
| DOI： |
| 中文关键词: Ammrjp1基因 PiggyBac 显微注射 转基因家蝇 |
| 英文关键词:Ammrjp1 gene PiggyBac microinjection transgenic housefly |
| 基金项目:广东省科技计划项目（2015B070701019）；广东省科学院科研平台环境与能力建设专项（2016GDASPT-0107）；国家科技支撑计划课题（2012BAD14B16） |
| Author Name | Affiliation | | XIONG Jia-Fu, ZHANG Jian-Qing, LIU Gui-Qing, HAN Ri-Chou* | 1. University of Chinese Academy of Sciences, South China Botanical Garden, Guangzhou 510650, China
2. Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou 510260, China |
|
| Hits: 1416 |
| Download times: 994 |
| 中文摘要: |
| 家蝇Musca domestica是一种重要的资源昆虫，作为饲料蛋白已广泛应用于动物养殖产业。MRJP1蛋白（Major Royal Jelly Proteins 1, MRJP1）是蜂王浆的主要蛋白成分，具有营养作用和跨物种促细胞增殖作用。构建表达意大利蜜蜂Apis mellifera王浆蛋白基因Ammrjp1的家蝇可望提高家蝇幼虫的应用价值。本研究构建了携带Ammrjp1基因的重组转座质粒pBac[fa PUb-mrjp1-DsRed]，显微注射家蝇胚胎，成功建立Ammrjp1转基因家蝇品系，RT-PCR证明Ammrjp1基因在转基因家蝇中正常转录；SouthernBlot证实Ammrjp1基因是以单拷贝的形式插入到家蝇基因组内；利用Inverse-PCR技术获得Ammrjp1基因在家蝇基因组上插入位点侧翼序列。与野生型家蝇比较，G8代Ammrjp1转基因家蝇4龄幼虫的百头重增加8.6%。家蝇遗传转化体系的成功构建，为建立新型转基因生物反应器和开发高值动物蛋白饲料提供技术支持。 |
| 英文摘要: |
| Housefly Musca domesticais an important resource insect, which is widely applied as feed protein in animal breeding industry. MRJP1 (Major Royal Jelly Proteins 1, MRJP1)is the main protein components in the honey bee royal jelly, with the function of nutrition and promotion of cell proliferation across species. With an aim to improve the application value of the housefly larvae, a transgenic housefly strain withAmmrjp1 gene inApis melliferawas constructed. In this study, the pBac[fa PUb-mrjp1-DsRed] recombinant plasmid withAmmrjp1 gene was constructed, and successfully microinjected into housefly embryos. RT-PCR showed that Ammrjp1 was normally transcribed. Southern Blot indicated that Ammrjp1was inserted into the housefly genome with a single copy. The insert site sequence of Ammrjp1 in the genome was cloned by Inverse-PCR. Compared to the non-transgenic houseflies, the weight of one hundred fourth instar G8 transgenic houseflies was 8.6% higher. This construction of genetic transformation system in housefly would provide technical support for the establishment of new transgenic bioreactors and the development of high-value animal protein feedstuffs. |
| View Full Text View/Add Comment Download reader |
| Close |
About Journal