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| 阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响 |
| Effect of sublethal concentrations of abamectin on protective and detoxifying enzymes inDiagegma semclausum |
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| DOI： |
| 中文关键词: 阿维菌素 半闭弯尾姬蜂 保护酶 解毒酶 |
| 英文关键词:Abamectin Diadegma semiclausum protective enzymes detoxifying enzymes |
| 基金项目:山西省自然科学基金项目（2012011035-3）；山西农业大学引进人才科研启动基金项目（XB2007008）；山西省研究生创新项目（20143091）；山西省大学生创新项目（2014097） |
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| 中文摘要: |
| 为明确阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响，采用试管药膜法以阿维菌素亚致死浓度（LC10 和 LC25）处理半闭弯尾姬蜂成虫，分别于处理后1、12、24和48 h测定其体内保护酶和解毒酶活性变化。结果表明，阿维菌素亚致死浓度处理后，随着时间的延长，对寄生蜂体内超氧化物歧化酶（SOD）和过氧化物酶（POD）活性总体表现为诱导作用；对过氧化氢酶（CAT）活性表现为先抑制后诱导作用；对酯酶（EST）、谷胱甘肽 S-转移酶（GST）和多功能氧化酶（MFO）具有显著的抑制作用。研究结果为深入了解半闭弯尾姬蜂对阿维菌素的防御机理提供了一定的理论基础。 |
| 英文摘要: |
| To explore the effect of abamectin on protective and detoxifying enzymes ofDiadegma semiclausum Hellen, a larval parasitoid of diamondback moth, the activities of protective and detoxifying enzymes were determined after treatment of D. semiclausumadults for 1, 12, 24 and 48 hours with LC10 and LC25 concentrations of abamectin using residual film method. The results showed that the activities of superxidase dismutase (SOD) and peroxidase (POD) were significantly promoted with increasing treatment time and the catalase (CAT) activity was firstly inhibited then promoted by sublethal concentrations of abamectin. At the same time, abamectin exposure inhibited the activities of detoxifying enzymes significantly at most of treatment time. The results provide some theory basis for further understanding defense mechanisms of D. semiclausum against abamectin. |
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