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| 大蜡螟气味受体基因Gmel/Orco的克隆与序列分析 |
| Gene cloning and sequence analysis of ( greater wax moth) the odorantreceptor gene Gmel /Orco in Galleria mellonella L. |
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| DOI： |
| 中文关键词: 大蜡螟 气味受体 基因克隆 序列对比 |
| 英文关键词:Galleria mellonella L. odorant receptors gene cloning sequence alignment |
| 基金项目:云南省农业科学院蚕桑蜜蜂研究所青年创新基金( QC2013003) |
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| 中文摘要: |
| 为进一步研究大蜡螟嗅觉通讯分子机制和寻求新的大蜡螟防治技术，本研究克隆了大蜡螟Galleria mellonella
L. 的气味受体基因Gmel /Orco，并对其序列进行生物信息学分析。根据GenBank 中已发表的鳞翅目昆虫非典型气味
受体家族基因的氨基酸保守序列设计简并引物，采用ＲT-PCＲ 方法扩增目的基因，将其克隆至T 载体并测序。克隆
获得大蜡螟气味受体Orco 的cDNA 序列，命名为Gmel /Orco ( GenBank 登录号: KT020861) ，序列分析结果显示，
Gmel /Orco 开放阅读框长1425 bp，编码474 个氨基酸，分子量为53. 36 kD，等电点为8. 44，序列中有7 个跨膜区，
N-端在细胞膜内，C-端在细胞膜外。通过在GenBank 中进行序列的同源性比较，该基因与已公布的鳞翅目螟蛾科、夜
蛾科昆虫的非典型气味受体基因序列有较高的同源性。克隆所获得的基因属于非典型气味受体家族基因。 |
| 英文摘要: |
| For further study the mechanism of olfactory communication and seek new prevention technology in Galleria mellonella L． ( greater wax moth) ，the olfactory receptor gene ( Gmel /Orco) was cloned，and analysed by bioinformatics analysis． According to the conserved amino acid sequences of the Lepidoptera insects olfactory receptor gene family published in GenBank，the degenerate primer were designed for ＲT-PCＲ． The cDNA sequence of wax moth olfactory receptor gene was cloned，and named Gmel /Orco ( GenBank accession number: KT020861) ，the results of sequence analysis showed that the open reading frame of Gmel /Orco consisted of 1425 nucleotides，encoding 474 amino acid with a molecular weight of 53.36 kD，an isoelectric point of 8.44 and 7 putative transmembrane domains in the sequence，with an intracellular N-terminal and extracellular C-terminus． By comparing the homology of the sequences in GenBank，the
sequence shared high identy with Orco orthologue chemoreceptor sequences from other previously reported insects. The result indicated that the gene belongs to atypical olfactory receptor gene family． |
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