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| 七星瓢虫滞育关联基因的转录组学分析 |
| Transcriptome analysis of diapause associated genes of Coccinella septempunctataunctata L. |
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| DOI： |
| 中文关键词: 七星瓢虫 滞育 转录组测序 滞育关联基因 KEGG分析、碳水化合物代谢 |
| 英文关键词:Coccinella septempunctataunctata L. diapause transcriptome sequencing diapause-associated genes KEGG analysis Carbohydrate metabolism |
| 基金项目:国家自然科学基金(31572062) ; 国家重点基础研究发展计划（973）项目 (2013CB127602) ; 公益性行业 (农业) 科研专项 (201103002) |
| Author Name | Affiliation | | QI Xiao-Yang, REN Xiao-Yun, AN Tao, CHEN Hong-Yin, HUANG Jian*, ZHANG Li-Sheng* | (1 Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100081, China) |
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| 中文摘要: |
| 对七星瓢虫正常发育、滞育以及滞育解除的雌成虫进行RNA测序，并对筛选出来的滞育相关基因进行KEGG通路富集分析，从分子水平解析七星瓢虫滞育发机理。本研究以正常发育产卵、滞育30 d以及滞育贮存30 d 后解除产卵的七星瓢虫雌成虫为研究对象，分别抽提RNA，合成cDNA，构建cDNA文库，文库检测合格后在Illummina Hiseq 2500测序仪上进行双向测序。根据测序结果，共获取unigene 82820个。采用两两比较法对正常发育组和滞育组、滞育组和滞育解除组进行差异表达分析，分别获得差异表达基因3501个和1427个。深入分析两组比对结果，将在滞育组上调且滞育解除组下调的unigene定义为滞育关联基因，共有443个基因为滞育关联基因。应用KEGG KAAS在线pathway比对分析工具对滞育关联基因进行通路富集分析，结果发现这些基因主要集中在碳水化合物代谢、脂质代谢以及信号转导等途径中。 |
| 英文摘要: |
| To understand the diapause mechanism of Coccinella septempunctataunctata L. at molecular level, transcriptome sequencing was carried out for female adults in three different states, i.e. , non-diapause(ND), diapause (D) and diapause terminated (DT), and KEGG pathway enrichment analysis was performed on the up-regulated expressed genes. In research, total RNA was respectively extracted from the female adults with normal development after oviposition, diapause 30 days, and diapause-terminated after diapause 30 days, and cDNA samples were synthesized to generate cDNA libraries. After quality inspection, two pair sequencing was performed with the Illumina HiSeq 2500 system. Total of 82820 unigenes were yieled according to sequencing results. When ND and D were compared, 3501 differentially expressed genes (DEGs) were revealed, and 1427 DEGs were detected between D and DT. The DEGs which was highly expressed in the diapause stage compared to non-diapause and diapause-terminated stages was defied as diapause-associated genes. In results, total of 443 unigenes were found to be diapause-associated genes. The analysis of KEGG enriched pathways were performed on diapause-associated genes using KAAS (KEGG Automatic Annotation Server) and found that these genes were mainly involved in carbohydrate metabolism, lipid metabolism and signal transduction |
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