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| 温度胁迫对小金蝠蛾幼虫抗氧化酶活性的影响 |
| Effects of temperature stress on antioxidant enzymes in Hepialus xiaojinensis (Lepidoptera: Hepialidae) larva |
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| DOI： |
| 中文关键词: 温度胁迫 小金蝠蛾 抗氧化酶 |
| 英文关键词:Temperature stress hepialus xiaojinensis antioxidant enzymes |
| 基金项目:国家自然科学基金项目（81173478） |
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| 中文摘要: |
| 小金蝠蛾 Hepialus xiaojinensis Tu. 是我国名贵中药材冬虫夏草的寄主昆虫之一，适宜在高海拔低温地区（8℃-12℃）生存。本文研究了小金蝠蛾在不同温度（4℃、12℃、16℃、20℃、24℃、28℃）条件下处理4 h和12 h后4龄幼虫体内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、谷胱甘肽硫转移酶（GSTs）的活力变化，以及总抗氧化能力（T-AOC）和丙二醛（MDA）含量的变化。结果表明，小金蝠蛾在正常生长发育情况下SOD具有较高活性；经温度胁迫后，SOD活力持续维持在较高活性，CAT、POD活性以及T-AOC 活性显著性升高（P <0.05）；GSTs在24℃以上活性有降低趋势，MDA含量有上升趋势。 |
| 英文摘要: |
| Hepialus xiaojinensis Tu., is a host insect of the precious Chinese herbal medicine, Ophiocordyceps sinensis . The larvae live in the soil and temperature seriously affects their survival . In this study, we investigated the activities of four antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GSTs), and peroxidase (POD), the variation of total antioxidant capacity (T-AOC) and the concentrations of malondialdehyde (MDA)under heat stress (0℃, 4℃, 12℃, 16℃, 20℃, 24℃ and 28℃) for 4 h and 12 h, respectively . The results showed that the activity of SOD kept a high level both in the control and treatment groups The activity of CAT, POD and T-AOC increased significantly after the temperature stress . The activity of GSTs decreased obviously and the concentrations of MDA increased when the treatment temperature exceeded 24℃. |
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