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| 基于线粒体COⅡ基因序列的中华蜜蜂地理种群的遗传多样性研究 |
| Genetic diversity of geographic populations of Apis cerana ceranaestimated by mitochondrial COⅡgene sequences |
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| DOI： |
| 中文关键词: 中华蜜蜂 线粒体COⅡ 地理种群 遗传多样性 遗传分化 |
| 英文关键词:Apis cerana cerana mtDNA COⅡ geographic population genetic diversity genetic differentiation |
| 基金项目:国家自然科学基金（31372382）；江苏省科技支撑项目（SBE201230535） |
| Author Name | Affiliation | | LIU Zhen-Guo1，JI Ting1*, SHEN Fang1, LIANG Qin2, LUO Yue-Xiong3 | (1. College of Animal Science and Technology, Yangzhou University,Yangzhou 225009, Jiangsu Province, China
2. College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3. Guangdong Entomological Institute, Guangzhou 510260, China) |
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| 中文摘要: |
| 通过分析我国20个不同地理种群中蜂线粒体COⅡ序列的变异，对中华蜜蜂群体遗传分化程度遗传多样性进行全面研究。结果表明：COⅡ基因部分序列中共发现16个变异位点和18个单倍型，核苷酸差异数的平均值为0.939，核苷酸分歧度（Dxy）在0.1%~0.965%之间变化，核苷酸遗传距离为-0.007%~1.489%。总种群的Fst为0.4978，差异均极显著(P<0.001)。研究结果显示种群总体单倍型多样性较为丰富，种群间核苷酸分歧度差异很大。20个东方蜜蜂不同地理种群间存在显著的遗传分化。 |
| 英文摘要: |
| To investigate the level of genetic diversity and genetic differentiation among different populations and within populations of Apis cerana cerana in China, partial sequences of mtDNA COⅡgene in 20 geographic populations from China were sequenced and analyzed. The result showed 16 variable sites and 18 haplotypes were observed in the sequence of mtDNA COⅡ. The average number of nucleotide divergence (K) of COⅡwere 0.939. Inter-population Nucleotide Divergence (Dxy) of COⅡwas ranged from 0.1％ to 0.965％. Inter-population Net Nucleotide Divergence (Da) in COⅡwas ranged from -0.007％ to 0.781％. The overall Fst value was 0.4978, which indicated the genetic variation was extremely significant within populations (P< 0.001). These results indicated that the haplotype diversity was relative abundance, the Dxy was very different. There was significant divergence among 20 A.cerana populations. |
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