| Xenorhabdus nematophila, a Gram-negative bacterium, is associated symbiotically with soil entomopathogenic nematodes, Steinernema carpocapsae, and is pathetic to a wide range of agriculture pests Several factors associated to the fermentation of X nematophila HB310 were measured and optimized in this study Glucose, beef extract-peptone and K2HPO4 were respectively chosen as the optimum carbon, nitrogen sources and inorganic salt using single factor method According to the orthogonal experiment, the optimal fermentation medium was identified as: 2% glucose, 1% beef extract-peptone, 0.3% beef extract and 1% K2HPO4 The optimized shake-flashing culture conditions were inoculum size of 6%, pH 7.5, 200 rpm rotary speed, 28℃and 48h fermentation time Under this condition, the X nematophila HB310 showed the fastest growth rate and the highest insecticidal activity |
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