《环境昆虫学报》

为了明确成虫密度、日龄和性别对应用药膜法测定杀虫剂毒力结果的影响，本文测定了敌百虫、高效氯氰菊酯和阿维菌素三种药剂对不同密度、不同日龄条件下雌雄橘小实蝇Bactrocera dorsalis (Hendel)雌雄成虫的毒力。结果表明，3种杀虫剂处理后不同密度桔小实蝇成虫的死亡率最高为62%-72%，之后逐渐下降低至10%以下，并趋于稳定；阿维菌素处理死亡率总体呈高-低-高-低的S形变化，以30-50头/瓶最高；高效氯氰菊酯处理后死亡率5头/瓶使最低，其他9个密度稳定在35.5%-46.4%；建立了描述3种药剂处理后死亡率和成虫密度之间关系的模型。同一种药剂处理不同日龄成虫，其死亡率变化规律明显。敌百虫处理4日龄、74日龄雌虫死亡率最高，14日龄最低；4、54、64日龄雄虫死亡率较高，14日龄最低；其雌雄虫死亡率与日龄件均符合Johnson Schumacher模型。高效氯氰菊酯处理雌雄虫的死亡率均以4日龄最低，之后明显增大，24日龄后趋于稳定；死亡率与日龄间的关系均符合Quardratics Ratio模型。同一日龄雌雄虫死亡率比较，敌百虫处理无明显差异，高效氯氰菊酯处理雌虫大多高于雄虫。根据以上研究，提出测定杀虫剂毒力时桔小实蝇的合适密度为0.04-0.06头/mL（容器体积）或者27.4-18.2 c㎡/头（药膜面积）。

英文摘要:

In order to elucidate the effects of testing density, age and gender on the results of dry film bioassay method, the toxicity of the trichlorphon, abamectin and β-cypermethrin to the female and male adults of oriental fruit fly Bactrocera dorsalis(Hendel)with different density and age were determined in this paper.The results showed that the mortality of B. dorsalis adults with different density were different after treated by the above 3 insecticides. The mortalities of adults with 5-10ind./bottle treated by trichlorphon was the highest as 62%-72%,then descreased gradually to below 10%，and tended to be stable .At the abamectin treatment ,the adult mortalities overall showed a S shape(eg.high-lou-high-low)change,and the top was with 30-50 ind./bottle.The adult mortality was the least when 5 ind./bottle,and varied from 35.5%-46.4%for the other 9 densities at cypermethrin treatment.The 3 models to describe the relateionships between the adult mortality and desity at the 3 insectticide treatments were presented.the adult mortalities with differeng day-old varied obviously when treated by the same insecticide, Treated after the trichlorphon,the mortalities 4 and 74 old male were the highest,and the least with 14 day-old ,and the mortalities for 4,54and 64 day-old males were higher while the lowest with 14 day-old. Johnson Schumacher models could be constructed to represent the relationships between the female/male age and the motality.At the cypermethrin treatment ,the mortalities of the 4 day-old male and female adults were the least,and then increased obviously,and tended to be stable after 24 day-old adults.Quadratics Ratio models were given to simulate the relationship between the mortality and the adult age .For the same day-old males and females ,their motalities with trichlorphon treatment were not significantly different ,but the ones for the females were mostly higher than those of the males for the cypermethrin treatment .According to the above results,the appropriate densities for the boassary of the the insecticides to B. dorsalis adult were put foward as 0.04-0.06 ind./mL(volume of container)or 27.4-18.2 cm2 per adult (membrane area).

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