三叶蔓荆提取物对几种害虫的生物活性研究
Study on the biological activity of extracts from Vitex trifolia against several insect pests
  
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中文关键词:  三叶蔓荆  甘蓝蚜  菜青虫  家蝇  生物活性
英文关键词:Vitex trifolia  Brevicoryne brassicae   Pieris rapae   Musca domestica   insecticidal activity
基金项目:广东省科技计划项目(2011B031500020、2012B020309007);广东省农业科学院院长基金(201406);广西农科院项目(桂农科2013YZ05)
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中文摘要:
      在实验室条件下研究了三叶蔓荆Vitex trifolia L叶片提取物对甘蓝蚜Brevicoryne brassicae L的拒食、对菜粉蝶Pieris rapae L幼虫的生长发育抑制以及对家蝇Musca domestica的毒杀活性。试验结果表明:10 mg/mL的乙酸乙酯提取物在24 h和48 h对蚜虫的拒食率分别为8889%和8636%,拒食效果明显。几种溶剂提取物对5龄菜粉蝶幼虫的生长发育均有不同程度的影响,其中乙酸乙酯提取物的体重增长抑制率第1天达7535%。各溶剂提取物在高浓度下对家蝇2龄幼虫均有一定的毒杀作用,低浓度下效果较差。乙酸乙酯提取物48 h的LC50为2278 mg/mL,远高于拒食和生长发育抑制试验设定的浓度。结果表明乙酸乙酯提取物拒食活性和生长发育抑制活性显著。
英文摘要:
      Antifeeding, inhibiting and contact activity of extracts from V trifolia leaves against Brevicoryne brassicae, Pieris rapae and Musca domestica were examined under laboratory conditions 10 mg/mL ethyl acetate extracts had bioactivity against B brassicae with antifeeding rate of 8889% and 8636% after 24 hours and 48 hours, which was conspicuous than other three extracts Four extracts had inhibiting effect against the fifth instar larvae of P rapae The activity of the ethyl acetate extract was significantly higher than that of other extracts with weight gain inhibitory rate of 7535% after 1 day Four extracts had insecticidal effect against the 2nd instar larvae of M domestica at high concentration After 48 hours, the LC50 of ethyl acetate extracts were 2278 mg/mL The results suggested that the ethyl acetate extracts of V trifolia leaves had high antifeeding and inhibiting activity to be studied in the future.
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