| In order to reveal the bacterial composition and their seasonal dynamics, we investigated the culturable bacteria in honeydew of Cinara tujafilina (del Guercio) collected from different months with culture-dependent method combined with 16S rRNA gene sequence analysis The results showed: (1) the quantity and diversity dynamics of culturable bacteria in honeydew was closely related to sampling season Overall, the bacterial quantity in summer and fall of 2011 showed great fluctuation, which reached maximum in July The bacterial diversity index of July sample also reached maximum in this year, followed by samples of October, August and September The bacterial quantity in spring of 2012 was relatively steady, which reached minimum in June The bacterial diversity index of June sample reached maximum in this year, followed by samples of May and April (2) Isolated bacterial strains from seven honeydew samples belong to 25 genera, and this showed that the honeydew of C tujafilina harbored abundant bacteria, among which, Bacillus, Curtobacterium and Agrobacterium showed obvious superiority in colony quantity As for seasonal distribution of bacteria, Bacillus occurred most frequently in honeydew, followed by Curtobacterium, Acinetobacter, Sphingomonas and Streptomyces (3) Phylogenetic analysis showed that 712% strains were Proteobacteria, 274% and 233% strains were Firmicutes and Actinobacteria respectively, and only one strain belonged to Bacteroidetes Although we cannot detect the symbiota in the honeydew, but we found that many other bacteria species exist in the honeydew of C tujafilina We will further compare the bacterial communities in the honeydew of different aphids, and discuss their possible roles playing in the reciprocal symbiosis between aphid-host tree-natural enemies. |
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