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| 两种体色生物型桃蚜对杀虫剂敏感性差异及其与酶活力的关系 |
| The sensitivity of two body colour bio-types of the green peach aphid Myzus persicae(Sulzer)(Hemiptera: Aphididae)to the pesticides and its correlation to enzyme activity |
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| DOI： |
| 中文关键词: 桃蚜 体色生物型 生物测定 酶活力 |
| 英文关键词:Myzus persicae(Sulzer) body colour bio-type bioassay enzyme activity |
| 基金项目:国家重点基础研究发展计划（2009CB119004）; 北京市科技计划项目（Z0906050060009017）;北京市科技新星计划（2010B027）;北京市优秀人才培养资助（2010D002020000010）;北京市农林科学院青年科研基金（QNJJ201214） |
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| 中文摘要: |
| 为了明确不同体色生物型桃蚜Myzus persicae (Suizer)对药剂的敏感性差异，本文采用室内生物测定和酶活力测定法检测不同体色生物型桃蚜对杀虫剂的敏感性以及与3种解毒酶和1种靶标酶活力关系。结果表明，红色生物型的敏感性明显低于绿色生物型，其中对吡虫啉的敏感性差异最大，LC50分别为6796648和284597 mg/L，红色为绿色生物型2388倍；其次为毒死蜱，LC50分别为12295798和1936816 mg/L，红色为绿色生物型635倍；两种生物型桃蚜对阿维菌素敏感性差异最小，红、绿色生物型的LC50分别为311678 和290966 mg/L，红色为绿色生物型107倍。红色生物型体内3种解毒酶（羧酸酯酶、谷胱甘肽-S-转移酶和多功能氧化酶P450）的比活力均高于绿色生物型，红色型羧酸酯酶比活力为绿色型的31倍，谷胱甘肽-S-转移酶为41倍，多功能氧化酶P450为15倍，两种体色生物型3种解毒酶的活力差异均达显著水平。两种体色生物型体内乙酰胆碱酯酶比活力没有差异，说明乙酰胆碱酯酶比活力与敏感性关系不大。 |
| 英文摘要: |
| In order to survey the difference of sensitivity to insecticides between the two body colour bio-types of the green peach aphid Myzus persicae(Sulzer)(Hemiptera: Aphididae), the bioassay was conducted in the laboratory, and three detoxifying enzymes and acetylcholinesterase activities of the two colour bio-types were testedThe results showed that the green bio-type was more sensitive to the insecticides than the red bio-typeThere was a significant difference in the sensitivity to the imidacloprid between the red bio-type and green bio-typeThe LC50 of red bio-type was 2388 times higher than that of the green bio-type with the value was 6796648 and 284597 mg/L, respectivelyThe difference of the sensitivity to chlorpyrifos between two body colour bio-types was moderateThe LC50 of red bio-type was 635 times higher than that of the green bio-type with the value was 12295798 and 1936816 mg/L, respectivelyThere was a little difference in the sensitivity to the abamectin between the two body colour bio-typesThe LC50 of red bio-type was 107 times as much as that of the green bio-type with the value was 311678 and 290966 mg/L, respectivelyThe esterase, glutathione-S-transferase and mixed function oxidase specific activity of red bio-type was extraordinarily higher than that of the green bio-typeThe esterase, glutathione-S-transferase and mixed function oxidase specific activity of the red bio-type were 31, 41 and 15 times as much as that of the green bio-type, respectively, whereas there was no difference in the acetylcholinesterase specific activity between the two body colour bio-types of Mpersicae, indicating that the acetylcholinesterase specific activity was not correlate with the sensitivity to the insecticides |
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