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| 苦瓜叶乙酸乙酯萃取物对斜纹夜蛾消化酶活性的影响 |
| Effects of ethyl acetate extracts of Momordica charantia leaves on digestive enzymes of Spodoptera litura(Fabricius) |
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| DOI：10.3969/j.issn.1674-0858.2012.03.04 |
| 中文关键词: 苦瓜 乙酸乙酯萃取物；Momordicin II 斜纹夜蛾 消化酶 |
| 英文关键词:Momordica charantia ethyl acetate extracts Momordicin II Spodoptera litura digestive enzymes |
| 基金项目:国家自然科学基金资助项目（31171849，30871646） |
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| 中文摘要: |
| 为了研究苦瓜Momordica charantia叶乙酸乙酯萃取物及其活性化合物Momordicin II对斜纹夜蛾Spodoptera litura的作用机理，将苦瓜叶乙酸乙酯萃取物及其活性化合物Momordicin II添加到人工饲料中饲喂斜纹夜蛾幼虫，分别测定其对斜纹夜蛾幼虫中肠蛋白酶活性和淀粉酶活性的影响。结果表明，斜纹夜蛾幼虫取食了含苦瓜叶乙酸乙酯萃取物的饲料后，其中肠的淀粉酶和蛋白酶活性均比对照明显降低，处理后72 h，高浓度（0.08%）对淀粉酶和蛋白酶的抑制率分别为23.0%和40.6%。斜纹夜蛾幼虫取食了含Momordicin II的饲料后，对淀粉酶活性无明显的影响，但其中肠的蛋白酶活性比对照明显降低，处理后72 h的抑制作用最强，浓度为0.6%时对蛋白酶的抑制率高达75.6%。这些结果说明乙酸乙酯萃取物及其活性化合物Momordicin II能够抑制和干扰斜纹夜蛾幼虫中肠的蛋白酶活性和淀粉酶活性，这是中毒斜纹夜蛾幼虫不能正常生长发育的原因之一。 |
| 英文摘要: |
| In order to understand the toxic mechanism of ethyl acetate extracts of Momordica charantialeaves and Momordicin II toSpodoptera litura. Artificial diet treated with ethyl acetate extracts of Momordica charantia leaves and Momordicin II to feed the 4th-instar larvae of Spodoptera litura, followed by analyzing the activities of amylase and protease of treated larvae, respectively. The results were shown that the activities of amylase and protease were decreased by ethyl acetate extracts of M.charantia leaves. After treating with extracts at 0.08% for 72 h, the inhibition rates of amylase and protease were 23.0% and 40.6%. After treated with Momordicin II, compared with control, there were no difference about the activities of amylase, but the activities of protease were markedly decreased, the inhibition effect of Momordicin II was very high after treating for 72 h, the inhibition rates of protease can reach up to 75.6% after treating at 0.6%. From these results, it was reasonably postulated that the ethyl acetate extracts of Momordica charantia leaves and Momordicin II disturbed and inhibited the activities of amylase and protease, this is the reason of larve of Slitura can not be normal growth and development. |
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