意大利蜜蜂工蜂中肠SNP和InDel突变位点的挖掘及分析
Exploration and analysis of single nucleotide polymorphism (SNP) and insersion-deletion (InDel) mutation loci in Apis mellifera ligustica worker’s midgut
投稿时间:2021-08-24  修订日期:2021-09-16
DOI:
中文关键词:  意大利蜜蜂  单核苷酸多态性  插入缺失突变  RNA-seq
英文关键词:Apis mellifera ligustica  SNP  InDel  RNA-seq
基金项目:国家现代农业产业技术体系建设专项资金项目(CARS-44-KXJ7);福建农林大学硕士生导师团队项目(郭睿); 江西省应用研究培育计划(20181BBF68003);福建省大学生创新创业训练计划(202010389016,202010389158)
作者单位地址
郭意龙 福建农林大学动物科学学院蜂学学院 福建农林大学动物科学学院蜂学学院
张文德 福建农林大学动物科学学院蜂学学院 
蔡宗兵 福建农林大学动物科学学院蜂学学院 
余岢骏 福建农林大学动物科学学院蜂学学院 
吴鹰 福建农林大学动物科学学院蜂学学院 
孙明会 福建农林大学动物科学学院蜂学学院 
康育欣 福建农林大学动物科学学院蜂学学院 
鲍佳益 福建农林大学动物科学学院蜂学学院 
徐细建 江西养蜂研究所 
陈大福 福建农林大学动物科学学院蜂学学院 
郭睿 福建农林大学动物科学学院蜂学学院 福建省福州市仓山区上下店路15号
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中文摘要:
      本研究利用已获得的意大利蜜蜂Apis mellifera ligustica工蜂中肠的转录组数据对意蜂的单核苷酸多态性(Single nucleotide polymorphism, SNP)和插入缺失(Insertion-Deletion, InDel)突变位点进行挖掘和分析,共鉴定到232 678个SNP位点,其中发生转换和颠换的SNP位点数分别为196 087和36 591个;最丰富的突变类型是G/A,最少的突变类型为T/G;分布在内含子的SNP位点最多,其次为外显子和基因间区;密码子突变类型为同义突变的SNP位点数最多,其次是非同义突变、终止子增加和终止子减少;此外,SNP位点所在基因可注释到50个GO条目和351条KEGG通路。共鉴定到38 715个InDel位点,最丰富的突变类型为移码插入,其次是移码缺失;分布InDel位点数较多的基因组区域为内含子和基因间区;另外,InDel位点所在基因可注释到49个功能条目和340条通路。研究结果丰富了西方蜜蜂的SNP和InDel位点信息,并为开发和利用意蜂的新型分子标记提供基础。
英文摘要:
      In this study, exploration and analysis of SNP (Single nucleotide polymorphism) and InDel (Insertion-Deletion) loci in Apis mellifera ligustica were conducted based on the obtained transcriptome data from Apis mellifera ligustica workers’ midguts. In total, 232 678 SNP loci were identified, among them 196 087 and 36 591 were transition and transversion, respectively; the most abundant mutation type of SNP loci was G/A, while the least type is T/G; SNP loci distributed in exons were the most, followed by exons and intergenic regions; SNP loci belonging to synonymous mutation were the largest, followed by non-synonymous mutation, stop gain and stop loss; additionally, genes containing SNP loci can be annotated to 50 GO terms and 351 KEGG pathways. Moreover, 38 715 InDel loci were identified, and the most abundant type was frameshift insertion followed by frameshift deletion; more InDel loci were distributed in introns and intergenic regions; in addition, genes containing InDel loci could be annotated to 49 functional terms and 340 pathways. These results not only enrich the information about Apis mellifera SNP and InDel loci, but also offer a basis for development and usage of novel molecular markers for Apis mellifera ligustica.
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