美国白蛾丝素蛋白基因的鉴定、时空表达及取食不同寄主植物后的表达响应
Cloning and spatio-temporal expression of fibroinsand its expression in response to feeding on different host in Hyphantria cunea ( Lepidoptera: Arctiidae)
投稿时间:2021-08-16  修订日期:2021-10-04
DOI:
中文关键词:  美国白蛾  寄主植物  丝素蛋白  时空表达特性
英文关键词:Hyphantria cunea  Host plants  Fibroins  temporal and spatial expression patterns  
基金项目:江苏省林业科技创新与推广项目(LYKJ[2020]17);江苏省研究生培养创新工程 ( KYCX18_0957) ;
作者单位地址
赵旭东 南京林业大学南方现代林业协同创新中心 江苏省南京市玄武区龙蟠路159号
韩芷琪 南京林业大学南方现代林业协同创新中心 
耿薏舒 南京林业大学南方现代林业协同创新中心 
胡天义 南京林业大学南方现代林业协同创新中心 
李文萱 南京林业大学南方现代林业协同创新中心 
郝德君 南京林业大学南方现代林业协同创新中心 江苏省南京市龙蟠路159号
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中文摘要:
      美国白蛾被列为我国林业检疫性害虫,其l-4龄幼虫幼虫具有吐丝结网幕的习性。为探究丝素蛋白基因的表达特性,本研究利用PCR技术克隆了美国白蛾的P25、Fib-H、Fib-L 3条丝素蛋白基因,并进行生物信息学分析;利用qPCR技术检测美国白蛾3条丝素蛋白基因的表达特性。结果表明:3条丝素蛋白基因序列比对均与车前灯蛾Arctia plantaginis丝素蛋白一致性最高,系统进化分析显示P25、Fib-H、Fib-L在鳞翅目不同科之间在出现较大的分化。RT-qPCR试验结果显示P25、Fib-H、Fib-L基因相对表达量与网幕产生高峰期(1、2龄)相一致。3种丝素蛋白均在丝腺组织中特异性高水平表达,在头部及脂肪体中有少量表达。此外,美国白蛾幼虫取食不同寄主植物后,3种丝素蛋白基因呈现不同的表达规律;取食杨树处理组中HcP25与HcFib-H基因相对表达量极显著高于取食其他寄主处理,而取食山樱花和日本晚樱处理组中HcFib-L基因表达量最高。研究结果为进一步探究丝素蛋白介导的美国白蛾对不同寄主的适应机制及其扩散机制奠定基础,也为开发美国白蛾防治新方法提供了潜在的基因靶标。
英文摘要:
      Hyphantria cunea Drury is are listed as quarantine pests in China, and its 1st-4th larvae aggregate by creating silk webs on tree branches, where the larvae feed and live as they grow. To explore thee xpression characteristics of silk fibroin genes. In this study, three silk fibroin genes were cloned from H. cunea by PCR and the results of sequence alignment showed that the silk fibroin genes of H. cunea had the highest consistency with those of Arctia plantaginis. Phylogenetic analysis showed that HcP25, HcFib-H and HcFib-L differentiated greatly among different families of Lepidoptera. RT-qPCR results indicated that the expression of HcP25, HcFib-H and HcFib-L in H.cunea in different developmental stages showed dynamic change and the expression level of HcP25, HcFib-H and HcFib-L was significantly related to the stage of silk-producing(1st and 2nd instars). RT-qPCR results in different tissues showed that HcP25, HcFib-H and HcFib-L were mainly expressed in silk glands and a small amount in head and fat body. Moreover, after feeding on different hosts, the expression trends of HcP25, HcFib-H and HcFib-L were different and the relative expression of HcP25 and HcFib-H genes of H. cunea larvae fed on poplar was significantly higher than that of other host populations, while the relative expression of HcFib-L fed on Cerasus serrulata(Lindl.) G. Done ex London var.lannesiana (Carri.) Makino and Cerasus serrulata (Lindl.) G.Done ex London was significantly higher than that of other hosts. The results of this study lay a foundation for in-depth study of web-mediated adaptation and diffusion mechanism of H. cunea to different hosts, and provide potential genetic targets for the development of new control methods of H. cunea.
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