曹联飞,苏晓玲,陈道印,赵东绪,华启云,2021,浙江省金华市中华蜜蜂线粒体 DNA 遗传多样性研究[J].环境昆虫学报,(4):986-991
浙江省金华市中华蜜蜂线粒体 DNA 遗传多样性研究
Genetic diversity of Apis cerana cerana based on mitochondrial DNA in Jinhua,Zhejiang,China
  
DOI:
中文关键词:  金华  中华蜜蜂  遗传多样性  线粒体 DNA
英文关键词:Jinhua  Apis cerana cerana  genetic diversity  mitochondrial DNA
基金项目:金华市农业研究计划 (2018-2-002) ; 浙江省农业 (畜禽) 新品种选育重大科技专项 ( 2016C02054-11) ; 浙江省农业重大技术协同推广计划 (2019XTTGXM03-02); 国家蜂产业技术体系 (CARS-44-SYZ7)
作者单位
曹联飞,苏晓玲,陈道印,赵东绪,华启云 1. 浙江省农业科学院畜牧兽医研究所杭州 310021
2. 金华市农业科学研究院浙江金华 321000 
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中文摘要:
      为了解浙江省金华市中华蜜蜂的遗传多样性及遗传分化现状,基于 mtDNA tRNAleu ~ COⅡ序列对金华市 9 个区县市共 81 个中蜂样本进行序列测定,分析了金华市中蜂群体的遗传多样性、遗传结构和遗传分化情况。结果共发现 14 个单倍型,其中主体单倍型 JH1 为 9 个地理群体所共享,与已报道的福建省中蜂主体单倍型以及浙江 省丽水市的中蜂主体单倍型相同。金华市中蜂的平均单倍型多样度为 0.610,平均核苷酸多样度为 0.00162,遗传多样性低于浙江省丽水市中蜂。分子变异分析 ( AMOVA) 显示,金华市中蜂不同地理群体间具有明显的遗传分化( FST = 0.212,P < 0.01) ,但大多数遗传变异存在于群体内 ( 78.79% ) ,群体间的遗传变异为 21.21% 。进一步分析发现金东区、浦江县与金华市其它中蜂地理群体之间存在显著的遗传分化,东阳市与金华市其它群体间的 遗传距离也较大。中性检验结果表明,金华市中蜂可能经历过群体扩张。相关研究结果对浙江省金华市中华蜜蜂的资源保护与合理开发利用具有重要意义。
英文摘要:
      In order to understand the genetic diversity and genetic differentiation of Apis cerana cerana in Jinhua,Zhejiang,China,the mitochondrial DNA tRNAleu ~ COⅡ sequences of 81 samples from each district and country of Jinhua were determined,and then the genetic diversity,genetic structure and genetic differentiation were analyzed. Fourteen haplotypes were found. The haplotype JH1 was shared by all nine districts and countries of Jinhua,which was identical to the main haplotype of A. cerana cerana in Fujian Province and Lishui,Zhejiang. It was shown that the haplotype diversity ( Hd) was 0.610 and the nucleotides polymorphism ( Pi) was 0.00162,which indicated that the genetic diversity of A. cerana in Jinhua was lower than Lishui. Analysis of molecular variance ( AMOVA) revealed significant genetic subdivision among populations ( FST = 0.212,P < 0.01 ) ,with 78.79% molecular variation within populations and 21.21% molecular variation among populations. Further analyses showed that significant genetic differentiations existed between A. cerana cerana from Jindong district and other populations of Jinhua. The differentiations of Pujiang country and other populations of Jinhua were also significant. The genetic distances between Dongyang and other populations of Jinhua were large. Neutral test showed that the population of A. cerana cerana in Jinhua might have experienced recent expansion. The results had important significance for the protection and rational development and utilization of A. cerana cerana in Jinhua,Zhejiang.
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