| 李文博，NAWAZ Haider Bashir，李新畅，田睿林，史树森，张 星，陈欢欢,2024,温度对短额负蝗成虫存活率及抗氧化酶活性的影响[J].环境昆虫学报,(6):1465-1472 |
| 温度对短额负蝗成虫存活率及抗氧化酶活性的影响 |
| Effect of temperature on the survival rate and antioxidant enzymes activity of Atractomorpha sinensis |
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| DOI： |
| 中文关键词: 短额负蝗 温度 抗氧化酶活性 总抗氧化能力 丙二醛 环境适应性 |
| 英文关键词:Atractomorpha sinensis temperature antioxidant enzyme activity total antioxidant capacity malondialdehyde environmental adaptability |
| 基金项目:云南省科技厅基础研究专项—青年项目（202301AU070005）；曲靖师范学院校级创新团队支持计划 |
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| 中文摘要: |
| 为明确不同温度对短额负蝗Atractomorpha sinensis成虫存活率以及体内抗氧化酶活性的影响。在24℃～40℃温度范围内，将短额负蝗健康成虫分组，分别置于生化培养箱处理4 h。测定各温度处理下成虫存活率和抗氧化酶（SOD、CAT、POD）的活性、总抗氧化能力（T-AOC）以及丙二醛（MDA）含量的变化。成虫存活率在24℃～34℃范围内均高于50%，其他温度处理成虫存活率明显降低。试验温度范围内短额负蝗体内3种抗氧化酶活性随温度升高呈先增后减趋势。32℃时雌、雄成虫的SOD、POD和CAT酶活值最高。T-AOC活性与MDA含量与抗氧化酶活性变化趋势相似，二者均在34℃时达最高。短额负蝗对不同温度的适应性存在差异，温度过高不利于其种群存活，此现象与其体内抗氧化酶活性密切相关。短额负蝗雌成虫抗氧化酶活性高于雄成虫，更能适应温度的变化。 |
| 英文摘要: |
| In order to clarify the effects of different temperatures on the survival rate of adult Atractomorpha sinensis and the activities of antioxidant enzymes in vivo. In the temperature range of 24℃～40℃, the healthy adults of A. sinensis were divided into groups and treated in biochemical incubator for 4 h. The survival rate of adults and the activities of antioxidant enzymes (SOD, CAT, POD), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were measured under different temperature. The survival rate of adults was higher than 50 % in the range of 24℃～34℃, and the survival rate of adults treated at other temperatures was significantly reduced. In the temperature range of the experiment, the activities of three antioxidant enzymes in the A. sinensis increased initially and then decreased with the increase of temperature. The activities of SOD, POD and CAT in female and male adults were the highest at 32℃. The changes of T-AOC activity, MDA content and antioxidant enzyme activity were similar, and both of them reached the highest at 34℃. There were differences in the adaptability of A. sinensis to different temperatures. Excessively high temperature was not conducive to its population survival, and was this phenomenon was closely related to its antioxidant enzyme activity. The antioxidant enzyme activity of female adults was higher than that of male adults. Females were more adaptable to temperature changes. |
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