| 梁文凯，刘莎，王玉琴，陈芬莲，朱家颖,2024,蠋蝽丝氨酸蛋白酶抑制剂基因鉴定及其表达特征分析[J].环境昆虫学报,(3):641-649 |
| 蠋蝽丝氨酸蛋白酶抑制剂基因鉴定及其表达特征分析 |
| Identification and expression profiling of serine protease inhibitor genes in Arma custos |
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| DOI： |
| 中文关键词: 捕食性蝽 蠋蝽 毒液 丝氨酸蛋白酶抑制剂 表达特征 |
| 英文关键词:Predatory bug Arma custos venom serine protease inhibitor expression profile |
| 基金项目:云南省农业基础研究联合专项重点项目（202101BD070001-024）；国家自然科学基金（32360686）；国家林草科技创新计划青年拔尖人才项目（2019132615）；云南省“兴滇英才支持计划”青年人才项目（YNWR-QNBJ-2018-393） |
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| 中文摘要: |
| 丝氨酸蛋白酶抑制剂（Serine protease inhibitor，SPI）是调控昆虫生长发育和免疫的关键因子，本文从蠋蝽Arma custos基因组中鉴定出12个SPI基因（AcusSPI1-12〖STBZ〗），并对其序列特征进行分析，采用逆转录-聚合酶链反应（Reverse transcription-polymerase chain reaction，RT-PCR）技术分析了SPI基因在成虫不同组织中的表达特征。结果表明，鉴定出的SPI基因可根据其作用机制分为serpin类和经典类（canonical SPI）。多序列比对结果表明，serpin类AcusSPI2-3与其它昆虫已知抑制性serpin铰链区的氨基酸残基是保守的，经典类AcusSPI7和AcusSPI8具有多个抑制结构域，AcusSPI12只具有单个抑制结构域，但它们都具有保守的半胱氨酸残基。RT-PCR结果显示，AcusSPI4、AcusSPI7和AcusSPI9基因在蠋蝽毒液器官中高表达，且在主腺前叶、主腺后叶和副腺中的表达存在差异。AcusSPI6和AcusSPI10基因在脂肪体中高表达。AcusSPI3在副腺、肠道和脂肪体中具有较高的表达量，AcusSPI10则〖STBZ〗在主腺后叶和脂肪体中高表达。研究结果，不仅表明蠋蝽部分SPI可作为毒液成分参与调控猎物免疫反应，而且为后期揭示它们的生理功能奠定了基础。 |
| 英文摘要: |
| Serine protease inhibitors (SPIs) play a crucial role in regulating insect growth, development and immunity. In this study, 12 SPI genes (AcusSPI1-12) were identified in Arma custos genome and their sequence characteristics were analyzed. The expression pattern of these SPI genes in different adult tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR). According to the action mechanism, the identified SPI genes could be classified into two types, serpin and canonical SPI. Multiple sequence alignment results indicated that the hinge region amino acid residues in AcusSPI2-3 of the serpin type were conserved with those of known inhibitory serpins from other insects. AcusSPI7 and AcusSPI8 belonging to the classical type had multiple inhibitory domains, while AcusSPI12 only had a single inhibitory domain. However, all two types had conserved cysteine residues. The RT-PCR results showed that AcusSPI4, AcusSPI7, and AcusSPI9 display differential expression profiles in different glands of the venom apparatus of A. custos, including anterior main gland, posterior main gland and accessory gland. AcusSPI6 and AcusSPI10 were highly expressed in the fat body, while AcusSPI3 was highly expressed in the accessory gland, gut and fat body. Additionally, AcusSPI10 was predominantly expressed in the posterior main gland and fat body. These results suggest that certain SPIs of A. custos may function as venom components to regulate the immune response of its prey, which lay the foundation for further investigating the physiological functions of them. |
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