| 吴福中，林秀莲，傅卫民，李惠萍,2024,榕属植物上9种粉蚧雌成虫的显微特征和28S rRNA分子鉴定[J].环境昆虫学报,(2):515-523 |
| 榕属植物上9种粉蚧雌成虫的显微特征和28S rRNA分子鉴定 |
| Microscopic characteristics of female adults and 28S rRNA molecular identification of 9 species of mealybugs in Ficus spp. |
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| DOI： |
| 中文关键词: 榕属植物 粉蚧 显微特征 28S rRNA 分子鉴定 |
| 英文关键词:Ficus spp. mealybugs microscopic characteristics 28S molecular identification |
| 基金项目:深圳技师学院科研项目（2314001）；惠州市科技项目（2017C0424040）；广东省科技计划项目（2019A141401001）；广东省科技奖入库培育项目（2021C0405004） |
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| 中文摘要: |
| 为明确榕属植物上粉蚧发生危害的种类，本文应用形态学和分子生物学方法，对榕属植物上调查的粉蚧开展鉴定。通过制作粉蚧雌成虫的玻片标本，从触角、刺孔群、盘孔和管腺等部位详细比较了9种粉蚧的主要显微特征，并制作了检索表。通过28S rRNA基因片段，扩增出38条序列，并与GenBank数据库进行相似性比对，相似度达到99.35%~100%，构建系统进化树，每种粉蚧与GenBank数据库下载的已知序列单独聚在一支，系统进化树结果与形态学鉴定结果一致，结果表明28S rRNA基因片段鉴定粉蚧具有可行性。该方法为农林业监测预警和精准防治粉蚧类害虫提供科学依据。 |
| 英文摘要: |
| By making slides of female adultmealybugs, we compared from the antennae, cerarii, disk pores and cylindrical ducts of the main microscopic characteristics, respectively, and made identification key. 38 sequences of 28S rRNA gene fragment were amplified, and compared with the GenBank database, the similarity reached 99.35%~100%.The phylogenetic tree was constructed with each mealybug was clustered together with the known mealybug sequences which downloaded from GenBank database separately, and the results of molecular identification were the same as morphological identification.The results showed that 28S rRNA gene fragment identification of mealybugs was feasible, and the method provided scientific basis for monitoring and warning and precise control of mealybugs in agriculture and forestry. |
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