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| 基于转录组测序技术研究黑水虻幼虫对锌离子胁迫的响应 |
| Response of Hermetia illucens L. to zinc ion stress based on transcriptome sequencing technology |
| 投稿时间：2024-01-30 修订日期：2024-06-07 |
| DOI： |
| 中文关键词: 黑水虻幼虫 锌胁迫 转录组 差异基因 信号通路 |
| 英文关键词:Hermetia illucens larvae zinc stress transcriptome differentially expressed genes signal pathways |
| 基金项目:国家自然科学(31260528,32060127) |
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| 中文摘要: |
| 黑水虻是世界范围内重要资源昆虫,过量锌离子(Zn2+)胁迫会对其幼虫的生长发育产生影响,而转录组分析有助于揭示Zn2+胁迫对黑水虻幼虫的分子响应机制。本试验以不同浓度Zn2+(75、150、300、1 200 mg/kg)对黑水虻幼虫进行连续5代胁迫,利用Illumina HiSeq 4 000高通量测序平台对第5代6龄幼虫转录组进行测序及分析。结果显示,Zn2+胁迫影响了第5代黑水虻幼虫的基因表达水平,75、150、300和1 200 mg/kg Zn2+处理幼虫中上调/下调的差异表达基因分别是1 293/1 839、1 277/2 306、1 080/1 966和1 444/2 170个；GO富集分析结果表明,差异表达基因在细胞过程中所占比例最大,细胞组分中主要是细胞和细胞部件,分子功能中主要为催化活性和细胞结合；KEGG富集差异分析显示,75 mg/kg Zn2+胁迫下差异表达基因主要富集在免疫相关反应、免疫相关信号传导途径及脂类物质代谢,300 mg/kg处理组差异表达基因主要富集在氨基酸代谢和碳水化合物代谢；1 200 mg/kg胁迫下氨基酸和脂类代谢、免疫相关反应和生长均受到严重影响,这些富集的与上述有关的差异基因大多表达下调。本研究表明Zn2+胁迫影响了黑水虻幼虫差异基因的表达,高浓度Zn2+胁迫差异基因表达影响尤为显著,主要响应在细胞过程、催化活性和结合的基因表达,且表达多为下调；高浓度Zn2+胁迫还导致了黑水虻幼虫免疫系统、环境相关信号传导途径和脂质代谢等的紊乱。 |
| 英文摘要: |
| Hermetia illucens is an important resource insect in the world. Excessive zinc ion (Zn2+) stress will affect the growth and development of its larvae, and transcriptome analysis will help to reveal the molecular response mechanism of Zn2+ stress to black hydra larvae. In this study, different concentrations of Zn2+ (75, 150, 300, 1 200 mg/kg) were used to stress the larvae of Black Hydra for five generations in a row, and the transcriptome of the 5th generation and 6th instar larvae was sequenced and analyzed by Illumina HiSeq 4 000 high-throughput sequencing platform. The results showed that Zn2+ stress affected the gene expression level of the fifth generation of Hermetia illucens larvae. The differentially expressed genes in the larvae treated with 75、150、300 and 1 200 mg/kg Zn2+ were 1 293/1 839, 1 277/2 306, 1 080/1 966 and 1 444/2 170 respectively. The results of GO enrichment analysis show that the differentially expressed genes account for the largest proportion in the cell process, and the cell components are mainly cells and cell components, and the molecular functions are mainly catalytic activity and cell binding. KEGG enrichment differential analysis showed that the differentially expressed genes were mainly enriched in immune-related reactions, immune-related signal transduction pathways and lipid metabolism under 75 mg/kg Zn2+ stress, while the differentially expressed genes in 300 mg/kg treatment group were mainly enriched in amino acid metabolism and carbohydrate metabolism. Under the stress of 1 200 mg/kg, amino acid and lipid metabolism, immune-related reaction and growth were seriously affected, and most of these enriched differential genes related to the above were down-regulated. Therefore, Zn2+ stress affected the expression of differential genes in the larvae of Hermetia illucens, especially the high concentration of Zn2+ stress, which mainly responded to cell process, catalytic activity and combined gene expression, and the expression was mostly down-regulated; High concentration of Zn2+ stress also led to the disorder of the immune system, environment-related signal transduction pathways and lipid metabolism of the larvae of Hermetia illucens. |
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