| 曾文慧，沈丹霓，吴文静，张世军，周虹，李志强,2024,Cflac1基因的RNAi对白蚁肠道氧化还原电位响应病原菌感染的影响[J].环境昆虫学报,(1):184-193 |
| Cflac1基因的RNAi对白蚁肠道氧化还原电位响应病原菌感染的影响 |
| The effect of RNA interference of Cflac1 gene on the response of termite intestinal redox potential to pathogenic infection |
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| DOI： |
| 中文关键词: 台湾乳白蚁 解毒酶 金龟子绿僵菌 肠道微环境 RNA干扰 |
| 英文关键词:Coptotermes formosanus detoxifying enzyme Metarhizium anisopliae intestinal microenvironment RNA interference |
| 基金项目:国家自然科学基金青年项目（32101536） |
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| 中文摘要: |
| 台湾乳白蚁Coptotermes formosanus又称为地下白蚁，它以木质纤维素为主食，是广泛分布于全球的经济害虫。地下白蚁对病原微生物强大的免疫防御能力一直是其生物防治应用的瓶颈之一。植食性昆虫的解毒酶在其消化和免疫调节中起着至关重要的作用。针对具有木质素代谢活性的解毒酶在白蚁肠道免疫过程中氧化还原稳态的调控研究对揭示其肠道免疫机制有重要意义。本研究以金龟子绿僵菌Metarhizium anisopliae为免疫诱导物，基于肠道氧化还原态的微电极检测系统，首次对台湾乳白蚁感染真菌后前肠/唾液腺、中肠及后肠中氧化还原电位的变化进行了描述；并利用RNA干扰技术，抑制了具有木质素代谢活性的乳白蚁漆酶基因Cflac1在感染前后的表达，并确定了Cflac1在乳白蚁肠道免疫防御真菌过程中对氧化还原稳态维系的重要作用。本研究丰富了白蚁肠道免疫机制的研究内容，为拓宽白蚁防治新靶点及生物防治技术的研发提供了理论支撑。 |
| 英文摘要: |
| Coptotermes formosanus Shiraki, a wood-feeding subterranean termite, is one of a globally distributed economic pests. Biocontrol of subterranean termites is largely impeded by their immune defenses. The detoxifying enzymes of phytophagous insects play a crucial role in their digestion and immune regulation. The study on the regulation of redox homeostasis by cletoxifying enzymes with lignin metabolism activity in the intestinal immune process of termites is of great significance for revealing the intestinal immune mechanism of termites. Here, Metarhizium anisopliae was using as an immune inducer. The variations in redox potential in the foregut/salivary gland, midgut, and hindgut of pathogen infected termites was firstly analyzed by using a microelectrode detection system. By using RNA interference (RNAi) technology, the expression of the laccase gene Cflac1, which was involved in lignin metabolism activity, was inhibited in the infected/uninfected termites. And the importance of Cflac1 in maintaining redox homeostasis in the intestinal immune defense against fungi in C. formosanus was also determined. This study enriches the research content on the intestinal immune mechanism of termites, providing theoretical basis for expanding new targets for termite control and the development of biological control technologies. |
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