《环境昆虫学报》

作者	单位
杨捷梅，莫东君，何漪婷，林嘉凝，汤婷，余小强，胡启豪	华南师范大学生命科学学院，广东省昆虫发育生物学与应用技术重点实验室，广州 510631

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中文摘要:

射精球蛋白EBP主要由射精管释放，是果蝇精液的重要组成部分。但是，EBP的具体作用机制及其在果蝇其它生命活动中的功能仍不清楚。本文检测了果蝇Ebp和EbpII基因在不同发育阶段以及成虫精巢与卵巢中的表达水平。结果发现，Ebp和EbpII基因在成虫中的表达水平显著高于其它发育阶段的果蝇，且它们在精巢中的表达显著高于卵巢。生物信息学分析表明，EBP和EBPⅡ蛋白仅包含若干低复杂区域，不含特殊的结构域，且它们的理化性质存在差异。在精细胞中特异性敲降Ebp后，果蝇储精囊中缺乏成熟的精子，但敲降EbpII后精巢没有显著的表型变化。TUNEL实验结果显示，在精细胞和生殖干细胞中特异性敲降Ebp的表达均能引起精子束的异常凋亡。由此，推测Ebp基因参与果蝇精子发生过程，并在维持精细胞存活过程中起关键作用。

英文摘要:

The ejaculatory bulb protein (EBP) produced by ejaculatory duct is the major component of seminal fluid in Drosophila melanogaster; however, its molecular mechanism and role in the development of other tissue are still largely unknown. In this paper, we analyzed the expression profile of Drosophila Ebp and EbpII in different developmental stages and in the testis and ovary of adult flies by qRT-PCR. The results showed that the expression of Ebp and EbpII transcripts was significantly higher in adult flies than in any other developmental stages, and both Ebp and EbpII mRNAs were expressed in the testis while their mRNA levels in the ovary were extremely low. Bioinformatics analysis showed that EBP and EBPⅡ proteins do not contain any known functional domains except a few low complex regions, and the physical and chemical characteristics between EBP and EBPII were different. When the expression of Ebp was knocked down by the spermatogonia specific Gal4 driver, the number of mature sperm in the seminal vesicle was significantly decreased compared to the control, while no apparent difference in the phenotype of testis was observed when EbpII was knocked down by RNAi. The results of TUNEL assay showed that several sperm bundles died when the expression of Ebp was knocked down by the spermatogonia and germ stem cell specific Gal4 drivers. Our results suggest that Ebp gene might participate in the spermatogenesis and contribute to maintain survival of sperm in Drosophila.

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