| 俞虹，高波，徐进，刘建宏，李永和,2023,基于转录组测序的楚雄腮扁叶蜂飞行肌蛋白及飞行能量代谢相关酶挖掘[J].环境昆虫学报,45(5):1333-1346 |
| 基于转录组测序的楚雄腮扁叶蜂飞行肌蛋白及飞行能量代谢相关酶挖掘 |
| Transcriptome-based discovery of flight muscle proteins and flight energy metabolism enzymes in Cephalcia chuxiongica |
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| DOI： |
| 中文关键词: 楚雄腮扁叶蜂 飞行肌显微结构 飞行肌结构蛋白 能量代谢酶 飞行能量代谢途径 |
| 英文关键词:Cephalcia chuxiongica flight muscle microstructure flight muscle structural proteins energy metabolism enzymes flight energy metabolism pathways |
| 基金项目:云南省农业基础研究联合专项重点项目（2018FG001-002）；云南省基础研究重点项目（202201AS070025） |
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| 中文摘要: |
| 本研究旨在阐明楚雄腮扁叶蜂Cephalcia chuxiongica Xiao飞行肌结构蛋白及飞行能量代谢相关酶基因信息。首先采用电镜技术对楚雄腮扁叶蜂飞行肌进行了观察，结果表明，肌原纤维（由粗丝和细丝组成，其数量比例为1∶3）是飞行肌的基本成分，肌原纤维横切面平均面积为0.771±0.042 μm^2，肌节平均长度为2.698±0.116 μm。进一步通过飞行肌转录组分析，共筛选到11大类飞行肌结构蛋白，其中肌球蛋白（Myosin）和肌动蛋白（Actin）的转录本数较多，分别为127个和54个。发现1个飞行蛋白Flightin编码基因（可编码一个长152 aa的蛋白），在该蛋白中检测到6个模体结构（Motif），膜翅目昆虫中在Motif 1的一些位点上其氨基酸类型表现出明显的特异性。在楚雄腮扁叶蜂中筛选到了25个飞行能量代谢关键酶编码基因，并基于这些酶构建了三条飞行能量代谢途径，即糖酵解（涉及15种酶）、脂肪氧化（涉及6种酶）和脯氨酸氧化（涉及4种酶）。在楚雄腮扁叶蜂中发现4个3-磷酸甘油脱氢酶GPDH异构体，但并未在这些异构体中找到类似果蝇飞行肌GPDH异构体所具有的特异C端三联肽（QNL），表明GPDH的进化存在物种特异性。 |
| 英文摘要: |
| The purpose of this study was to elucidate the gene information of flight muscle structural proteins and flight energy metabolism enzymes in Cephalcia chuxiongica. Firstly, the microscopic structure of the flight muscle was observed by electron microscopy. The results showed that myofibrils were the basic components of flight muscle. Myofibrils were composed of thick filaments and thin filaments, with a quantity ratio (thick filaments∶thin filaments) being 1∶3. The mean myofibril cross-section area was 0.771±0.042 μm^2 and the mean sarcomere length was 2.698±0.116 μm. Through flight muscle transcriptome analysis, 11 categories of flight muscle structural proteins were identified, of which myosin and actin had larger numbers (127 and 54, respectively) of transcripts. A gene encoding the Flightin (152 aa) was also found in C. chuxiongica. Six motifs were detected in Flightin, and hymenopterans showed obvious specificity in amino acid types at some positions in Motif 1. A total of 25 flight energy metabolism enzyme encoding genes were found in C. chuxiongica. Three flight energy metabolism pathways were constructed based on these enzymes, namely glycolysis (15 enzymes involved), fat oxidation (6 enzymes involved) and proline oxidation (4 enzymes involved). In this study, four glycerol-3-phosphate dehydrogenase (GPDH) isoforms were found in C. chuxiongica. However, the specific C-terminal tripeptide (QNL) of Drosophila flight muscle GPDH isoform has not been found in the isoforms of C. chuxiongica, indicating the evolution of GPDH is species-specific. |
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