白旭,冯嘉伟,白新宇,李庆荣,钟仰进,杨婉莹,2023,高低温催青温度敏感期家蚕卵的差异蛋白筛查[J].环境昆虫学报,(4):997-1008
高低温催青温度敏感期家蚕卵的差异蛋白筛查
Differential protein screening of silkworm eggs in temperature-sensitive period of high and low temperature
  
DOI:
中文关键词:  家蚕  滞育  温度敏感期  差异蛋白
英文关键词:Silkworm  diapause  temperature-sensitive period  differential protein expression
基金项目:广东省基础与应用基础研究基金(2021A1515012090)
作者单位
白旭,冯嘉伟,白新宇,李庆荣,钟仰进,杨婉莹 1. 华南农业大学动物科学学院, 广东省农业动物基因组学与分子育种重点实验室, 广州 510642
2. 广东省农业科学院蚕业与农产品加工研究所, 广州 510610 
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中文摘要:
      滞育(Diapause)是昆虫发育停滞或减缓的生理状态,家蚕Bombyx mori作为卵滞育的代表,其滞育过程已得到广泛研究,但诱导滞育发生的分子机制尚不清楚。二化性家蚕滞育性由遗传和母系在胚胎期所处的环境条件决定,25℃催青蚕卵孵化后的家蚕产滞育卵,15℃低温催青则诱导家蚕产下非滞育卵。本研究分别用25℃和15℃催青蚕卵,在发生滞育诱导的温度敏感期取样,抽提蛋白质通过非标(Label-free)蛋白质组定量技术进行质谱测序。筛选出具有明显表达差异的蛋白104个,其中56个蛋白上调,48个蛋白下调。通过生物信息学对差异蛋白进行GO分类和KEGG功能富集分析,结果显示差异蛋白主要参与生长发育、物质代谢和胁迫应答等生物过程;同时差异蛋白主要参与胰岛素信号通路、乙醛酸和二羧酸代谢等相关途径。分别选取上调基因和下调基因进行qRT-PCR验证,其趋势与蛋白组学结果一致。该研究将为进一步解析家蚕滞育诱导发生机制提供靶标蛋白和数据参考。
英文摘要:
      Diapause is a physiological state of stagnation or retardation of insect development. Bombyx mori, as the representative of egg diapause, its diapause process has been widely studied, but the molecular mechanisms that induce diapause are still unclear. The diapause of bivoltine silkworm is determined by genetic and matriarchal in embryonic period environment condition, 25℃ after incubation eggs hatch of silkworm production diapause eggs, 15℃ induction of silkworm lay non-diapause eggs. In this study, the eggs were incubated at 25℃ and 15℃ respectively, and the samples were taken during the temperature-sensitive period of diapause induction. The extracted proteins were sequenced by mass spectrometry using label-free proteome quantitative technology. 104 proteins with obvious expression differences were screened, among which 56 proteins were up-regulated and 48 proteins were down-regulated. GO classification and KEGG functional enrichment analysis of the differential protein expression by bioinformatics showed that differential proteins were mainly involved in biological processes such as growth and development, material metabolism and stress response. Differential protein expression were mainly involved in the insulin signaling pathway, glyoxylic acid and dicarboxylic acid metabolism and other related pathways. Up-regulated genes and down-regulated genes were selected for qRT-PCR verification, and the trend was consistent with proteomics results. The results will provide target proteins and data references for further analysis of the mechanism of diapause induction in silkworm.
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