| 李婷，曹玉峰，王常禄，徐业，李伟军，林莉，Abdul Hafiz Ab Majid，王松灌，王建国,2023,臭虫击倒抗性基因突变检测及抗性测定[J].环境昆虫学报,(3):731-739 |
| 臭虫击倒抗性基因突变检测及抗性测定 |
| Detection of knockdown resistance gene mutation and evaluation of insecticide resistance of bed bugs (Cimex spp.) |
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| DOI： |
| 中文关键词: 温带臭虫 热带臭虫 击倒抗性基因 |
| 英文关键词:Cimex lectularius Cimex hemipterus knockdown resistance gene |
| 基金项目:江西省双千人才项目(G202000222010)；国家外国专家项目(G20200222010，G2021022002);外交部重点项目（新F2023） |
| 作者 | 单位 | | 李婷，曹玉峰，王常禄，徐业，李伟军，林莉，Abdul Hafiz Ab Majid，王松灌，王建国 | 1. 江西农业大学入侵生物实验室，南昌 330045；2. Department of Entomology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
3. Household and Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
4. Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah Malaysia |
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| 中文摘要: |
| 近20多年，臭虫（Cimex spp.）在世界范围内成为常见的卫生害虫，其防治主要采用化学防治，但很多种群发现击倒抗性（Knockdown resistance gene, kdr）基因突变的存在以及抗药性。监测kdr的发生频率以及不同种群对农药的抗性对臭虫有效防治很重要，但我国对臭虫种群的抗药性报道很少。本试验采用点滴法测定了1个温带臭虫Cimex lectularius野外种群对氯虫苯甲酰胺、呋虫胺、吡虫啉、噻虫嗪和高效氯氰菊酯等5种药剂的毒性及抗性水平，使用区分剂量快速鉴定抗性方法对2个温带臭虫和2个热带臭虫Cimex hemipterus种群对高效氯氰菊酯的抗性水平进行了检测，此外用PCR方法检测8个臭虫地理种群（1个温带臭虫实验室种群，1个温带臭虫野外种群和6个热带臭虫野外种群）174个个体的kdr突变频率。点滴法结果表明，5种杀虫剂对温带臭虫的毒性是吡虫啉和呋虫胺>噻虫嗪>氯虫苯甲酰胺和高效氯氰菊酯，测试的野外温带臭虫种群仅对噻虫嗪无明显抗性。热带臭虫2个野外种群对高效氯氰菊酯的抗性均远高于温带臭虫。在温带臭虫的实验室种群中未检测到突变，在野外种群中检测到了V419L和L925I突变，可分为2种基因型类型（A：无突变位点；B：同时有L925I和V419L），而在热带臭虫的6个种群检测到M918I和L1014F突变，只有1种基因型类型，即M918I和L1014F双位点突变。温带臭虫1个野外种群及热带臭虫6个野外种群kdr突变的存在与臭虫对高效氯氰菊酯敏感密切关联。基于kdr基因突变检测结果推测我国臭虫种群广泛存在对拟除虫菊酯的抗性。 |
| 英文摘要: |
| Bed bugs (Cimex spp.) experienced global resurgence during the past 20 years and have become common urban pests. Insecticide application is the primary control method for these pests. But the presence of knockdown resistance gene (kdr) mutations and insecticide resistance have been detected in many bed bug populations. Monitoring the occurrence frequency of kdr and insecticide resistance in different populations is important for effective control of bed bugs. However, there are few reports on bed bug resistance in China. In this study, the toxicity and resistance levels of a field population of Cimex lectularius to Chlorantraniliprole, Dinotefuran, Imidacloprid, Thiamethoxam and Beta-Cypermethrin were determined by topical assay. We assessed resistance to Beta-Cypermethrin in two populations of C. lectularius and two populations of C. hemipterus using a discriminating dose. In addition, PCR method was used to detect the kdr mutation frequency of 174 individuals from eight geographic populations (one laboratory C. lectularius, one field C. lectularius populations, and six C. hemipterus field populations）. The results of the topical assays indicated that susceptibility of C. lectularius to five insecticides was: Imidacloprid and Dinotefuran>Thiamethoxam>Chlorantraniliprole and Beta-Cypermethrin. The field C. lectularius population developed resistance to all tested insecticides except thiamethoxam. The resistance to Beta-Cypermethrin of the two field populations of C. hemipterus was higher than that in C. lectularius. No mutations were detected in laboratory populations of C. lectularius, and V419L and L925I mutations were detected in wild populations, they can be divided into two genotypic types (A: no mutated sites; B: L925I and V419L）. However, M918I and L1014F mutations were detected in six populations of C. hemipterus, and there was only one genotype, namely M918I and L1014F double-locus mutations. Existence of mutation sites was closely related to the susceptibility of bed bugs to a pyrethroid insecticide. Results suggested resistance to pyrethroids was widespread among the Chinese bed bug populations. |
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