| 王天宝，邱园妹，孙巧玲，杨长生，Aqai Kalan Hassanyar，黄景南，李志国，苏松坤,2023,ame-miR-124-3p对西方蜜蜂气味学习的影响[J].环境昆虫学报,(2):473-479 |
| ame-miR-124-3p对西方蜜蜂气味学习的影响 |
| Effects of ame-miR-124-3p on odour learning in the honey bee (Apis mellifera) |
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| DOI： |
| 中文关键词: 西方蜜蜂 气味学习 miRNAs 伸吻反应 抑制与过表达 大脑功能 |
| 英文关键词:Apis mellifera odour learning miRNAs proboscis extension response inhibition and overexpression brain function |
| 基金项目:国家现代农业产业技术体系（蜜蜂）项目（CARS-44-KXJ4） |
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| 中文摘要: |
| 越来越多的证据表明miRNA在神经调控中起着重要的作用。本研究选取12日龄的西方蜜蜂Apis mellifera，探讨蜜蜂个体脑部ame-miR-124-3p对气味学习的功能研究。首先对11日龄的蜜蜂分别饲喂ame-miR-124-3P抑制剂及其无意义序列、ame-miR-124-3P的模拟物及其无意义序列。饲喂24 h后对4组蜜蜂进行气味联想性学习实验，随后通过伸吻反应（Proboscis extension response，PER）比较4组蜜蜂的嗅觉学习差异。最后采用实时荧光定量PCR（qRT-PCR）验证干扰结果。PER结果显示，饲喂ame-miR-124-3P抑制剂的蜜蜂学习比率显著降低，饲喂ame-miR-124-3P模拟物的蜜蜂学习比例显著升高。荧光定量PCR（qRT-PCR）检测结果显示ame-miR-124-3P的表达水平在饲喂ame-miR-124-3P抑制剂组蜜蜂脑部显著降低（P<0.05），饲喂ame-miR-124-3P模拟物组蜜蜂脑部显著升高（P<0.05）。研究发现，蜜蜂脑部ame-miR-124-3P受到抑制后，蜜蜂的气味学习能力显著性降低，ame-miR-124-3P的过表达则会显著增强蜜蜂的气味学习能力，表明ame-miR-124-3P可能是调控蜜蜂学习行为的重要因子，研究结果有助于后续进一步研究蜜蜂气味学习行为相关的分子机制。 |
| 英文摘要: |
| Increasing researches evidence suggests that miRNAs play an important role in neuroregulation. In this study, 12 d bees Apis mellifera were selected to study the effects of ame-miR-12-4-3p on odour learning. At first, 11 d bees were selected and fed with ame-miR-124-3P inhibitor, ame-miR-124-3P inhibitor-NC, ame-miR-124-3P mimics, and ame-miR-124-3P-mimics. Odour association learning experiments were performed on the four groups of bee 24 h after feeding, and olfactory learning differences were subsequently compared by proboscis extension response (PER). Finally, quantitative real-time PCR (qRT-PCR) was conducted to verify the interference results. The PER results showed a significant decrease in the learning ratio of bees fed with ame-miR-124-3P inhibitors and a significant increase in the learning ratio of bees fed ame-miR-124-3P mimics. qRT-PCR results showed that the brain expression level of ame-miR-124-3P was significantly decreased in the bees fed with the ame-miR-124-3P inhibitor (P<0.05), and significantly increased in the bees fed with the ame-miR-124-3P mimics (P<0.05). In this study, inhibition of ame-miR-124-3P in bees brain significantly suppressed odour learning ability, and overexpression of ame-miR-124-3P significantly enhanced odor learning ability, suggesting that ame-miR-124-3P may be an important factor regulating bee learning ability, and the results contributes to further studies on molecular mechanisms related to odour learning in the bee. |
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