| 周丹，郅军锐，岳文波，张涛，李定银，刘利,2023,西花蓟马消化酶对食物短期适应的生化机制[J].环境昆虫学报,45(1):233-238 |
| 西花蓟马消化酶对食物短期适应的生化机制 |
| Biochemical mechanism of short-term adaptation of digestive enzymes in Frankliniella occidentalis to different foods |
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| DOI： |
| 中文关键词: 西花蓟马 食物 消化酶 酶活性 |
| 英文关键词:Frankliniella occidentalis food digestive enzymes enzyme activity |
| 基金项目:国家自然科学基金（32072424） |
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| 摘要点击次数: 219 |
| 全文下载次数: 286 |
| 中文摘要: |
| 为明确西花蓟马Frankliniella occidentalis消化酶对食物适应的生化机制,用玫瑰花瓣、玫瑰花朵（含花粉花蜜）和10%蜂蜜水+菜豆豆荚3种食物分别饲养西花蓟马雌成虫，测定西花蓟马在取食1、3、6、9、12、24和48 h后其雌成虫体内α-淀粉酶、胰蛋白酶和胰凝乳蛋白酶活性的变化。结果表明，取食玫瑰花朵的西花蓟马体内α-淀粉酶和胰蛋白酶活性最高，大多数时间下取食玫瑰花瓣的最低。随取食时间延长，除取食玫瑰花瓣的西花蓟马虫体α-淀粉酶是先降低后升高外，取食另外2种食物的α-淀粉酶和胰蛋白酶活性均是先升高后降低。西花蓟马体内胰凝乳蛋白酶的活性在3~6 h时取食玫瑰花朵的活性最低，在其余时间下酶活性的变化在3种食物间没有明显的规律。结果说明，西花蓟马可通过快速调节体内消化酶活性来适应不同食物的变化，达到自身生长发育对营养物质的需求。 |
| 英文摘要: |
| In order to explore biochemical mechanism of short-term adaptation of digestive enzymes in Frankliniella occidentalis to different foods, three foods of rose petal, rose flower (containing pollen and nectar)and 10% honey solution+kidney bean pod were used to feed female adults of F. occidentalis. The differences in the activities of digestive enzymes (α-amylase, trypsin and chymotrypsin)were compared after feeding the various foods at 1, 3, 6, 9, 12, 24 and 48 h, respectively. The results showed that the activities of α-amylase and trypsin were the highest in feeding rose flower at any time, and they were the lowest in thrips feeding rose petal at most time. The activity of α-amylase in thrips feeding rose petal firstly decreased and then increased with time extension, while it firstly rose to the higher level and then dropped when thrips fed other two foods. The activities of chymotrypsin were lowest when thrips fed rose flower at 3~6 h, and which hadn't obvious regularity at other time. The results of the study indicated that F. occidentalis could rapidly adjust the activities of digestive enzyme, to adapt different foods and achieve sufficient nutrients for growth and development. |
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