| 姚知含，李玲，庞保平，单艳敏，季彦华，刘东力,2023,沙葱萤叶甲对蜕皮激素响应的转录组分析[J].环境昆虫学报,45(1):155-162 |
| 沙葱萤叶甲对蜕皮激素响应的转录组分析 |
| Transcriptome analysis of the adult Galeruca daurica (Coleoptera: Chrysomelidae)in response to 20-hydroxyecdysone |
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| DOI： |
| 中文关键词: 沙葱萤叶甲 转录组 蜕皮激素 生殖滞育 代谢通路 |
| 英文关键词:Galeruca daurica transcriptome 20-hydroxyecdysone reproductive diapause metabolic pathways |
| 基金项目:国家自然科学基金项目（31760517） |
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| 摘要点击次数: 187 |
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| 中文摘要: |
| 为建立沙葱萤叶甲Gauleruca daurica成虫对蜕皮激素（20-hydroxyecdysone，20E）响应的转录组数据库，挖掘对20E响应的基因以及代谢和信号通路，并在转录组水平探讨20E调控生殖滞育的分子机制,本研究采用Illumina HiSeqTM4000高通量测序平台对20E及二甲基亚砜（dimethyl sulfoxide，DMSO）处理后的沙葱萤叶甲成虫进行了转录组测序，共获得80 313个unigene；与阴性对照DMSO相比，共获得201个差异表达基因，其中106个上调、95个下调。GO和KEGG富集分析表明，多数差异表达功能基因富集于各种代谢通路，其中核黄素代谢（riboflavin metabolism）、溶酶体（lysosome）和泛酸与乙酰辅酶A合成（pantothenate and CoA biosynthesis）通路显著富集（q<0.05）。结果表明，20E可能通过影响多种代谢通路调控沙葱萤叶甲的生殖滞育。 |
| 英文摘要: |
| This study aimed to establish the transcriptome database of the Gauleruca daurica adults in response to 20-hydroxyecdysone (20E), to excavate the genes and metabolic/signaling pathways in response to 20E, and to explore the molecular mechanism of 20E regulating reproductive diapause at the transcriptome level. According to the sequencing results from the 20E-and DMSO-treated adults of G. daurica on the Illumina HiSeqTM4000 platform, a total of 80 313 unigenes were obtained. Compared with those in the negative control, DMSO-treated adults, 201 unigenes in the 20E-treated adults were differentially expressed (106 up-regulated and 95 down-regulated). The GO and KEGG enrichment analysis showed that the majority of DEGs were enriched in various metabolic pathways among which riboflavin metabolism, lysosome, and pantothenate and CoA biosynthesis pathways were significantly enriched (q<0.05). These results showed that 20E might regulate reproductive diapause in G. daurica via various metabolic pathways. |
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