| 吴洪鑫，许亚婷，康泽泓，杨荣蓉，陆永跃，金丰良，许小霞,2022,红火蚁Serpin家族基因鉴定及其在绿僵菌侵染下的表达模式分析[J].环境昆虫学报,44(6):1541-1552 |
| 红火蚁Serpin家族基因鉴定及其在绿僵菌侵染下的表达模式分析 |
| Genome-wide identification of Serpin gene family in Solenopsis invicta and expression analysis in response to Metarhizium anisopliae |
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| DOI： |
| 中文关键词: 红火蚁 金龟子绿僵菌 丝氨酸蛋白酶抑制 免疫反应 |
| 英文关键词:Solenopsis invicta Metarhizium anisopliae Serpin immune responses |
| 基金项目:国家重点研发计划项目（2021YFD1000500） |
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| 中文摘要: |
| 丝氨酸蛋白酶抑制剂（Serine protease inhibitor, Serpin）在昆虫Toll通路和PPO级联反应的防御机制中起着至关重要的作用。本研究利用现代生物信息学工具预测和分析了红火蚁Solenopsis invicta中Serpin家族的结构和功能多样性。染色体定位表明，红火蚁基因组中7个Serpin基因家族成员不均匀的分布在4条染色体上。结构域分析表明,SiSerpin蛋白具有Serpin基因的保守结构，即典型的反应中心环（Reactive central loop, RCL）结构与Serpin结构域，不同成员蛋白拥有多样化的基序。系统进化分析表明，红火蚁Serpin蛋白与其他的昆虫Serpin蛋白有较高亲缘关系，SiSerpin7与烟草天蛾Manduca sexta的Serpin3a亲缘关系最近，SiSerpin12与黄粉虫Tenebrio molitor Serpin55亲缘关系最近，SiSerpin13与烟草天蛾Serpin6亲缘关系最近，SiSerpin4与家蚕Serpin27亲缘关系最近。为了进一步明确红火蚁Serpin家族在绿僵菌Metarhizium侵染后的表达模式，用绿僵菌侵染不同体型的工蚁，荧光定量PCR（RT-qPCR）分析表明，红火蚁Serpin基因家族成员能在早期响应绿僵菌的侵染；除SiSerpin7基因外，大型和小型两种工蚁的表达模式存在较大的差异。结果表明红火蚁Serpin家族作为免疫调控蛋白，能够响应绿僵菌的侵染，且在红火蚁不同体型工蚁之间存在着不同的免疫调控模式。这些发现不仅为Serpins的功能分析提供了理论基础，而且有可能有助于绿僵菌作为一种有效的生防剂的开发。 |
| 英文摘要: |
| Serpins play crucial modulatory roles in the insect defense mechanism, mainly in the Toll pathway and prophenoloxidase (PPO) cascade. In the current study, we employed modern Bioinformatics tools to predict and analyse the structural and functional diversity of Serpin family in Solenopsis invicta, an invasive and notoriously harmful species of ants. Computational analysis showed that Serpin family genes were evenly distributed on four chromosomes. Domain analysis revealed that S. invicta Serpin (SiSerpin) protein had the conserved structure, typical reactive central loop (RCL) and serpin domain, whereas different member proteins had diverse motifs. Phylogenetic analysis showed that the SiSerpin family had a strong relationship with Serpin genes from other insect species. SiSerpin7 was most closely related to Serpin3a of Manduca sexta, and SiSerpin12 was strongly related to Serpin55 of Tenebrio molitor. Similarly, SiSerpin13 was found to be in close homology with Serpin6 in M. sexta while SiSerpin4 was closely linked to Bombyx mori Serpin27. To further understand the role of SiSerpin in immune response, the S. invicta were infected with Metarhizium anisopliae, an insect pathogenic fungi. RT-qPCR results showed that the expression profiles of SiSerpin family greatly varied at the initial stages of infection, an indicator of their regulatory potential during fungal stress. It was worth mentioning that the expression patterns differed in small and large worker ants. These results presented an intricate immuno-regulatory potential of the Serpin family of S. invicta in response to the fungal infection. We also noted the difference in immune responses amongst different sizes of the ants. These findings not only provide a theoretical basis for the functional analysis of Serpins, but could potentially assist in the development of M. anisopliae as an effective biocontrol agent of S. invicta. |
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