《环境昆虫学报》

叉角厉蝽Eocanthecona furcellata是一种在生物防治方面有重要作用和潜力的捕食性天敌昆虫，触角是昆虫进行信息交换的重要器官，而嗅觉相关基因则是调控天敌昆虫捕食行为的重要分子基础。为获得叉角厉蝽触角转录组数据库，挖掘叉角厉蝽嗅觉相关基因，本研究利用Illumina高通量测序平台对叉角厉蝽雌雄成虫与5龄若虫触角进行转录组测序。成功构建了叉角厉蝽触角转录组，获得了67 843条unigenes，N50长度为2 300 bp。与七大公共数据库比对注释到27 686条unigenes，其中NR数据库注释最多(33.33%)，且与茶翅蝽Halyomorpha halys相似度最高(64.20%)。14 258条注释到GO数据库中，分为生物过程、细胞组分和分子功能3个大类42个亚类；KEGG代谢途径分析表明，7 703条形成282条代谢通路，其中被注释在信号传导通路中的unigenes最多(11.50%)。进一步基因注释分析，鉴定得到134个候选嗅觉相关基因，32个化学感应蛋白基因(Chemosensory protein genes，CSP)，10个气味结合蛋白基因(Odorant binding protein genes，OBP)，21个气味受体基因(Gustatory receptor genes，GR)，48个嗅觉受体基因(Olfactory receptor genes，OR)，17个离子型受体基因(Ionotropic receptor genes，IR)，6个感觉神经元膜蛋白基因(Sensory neuron membrane protein genes，SNMP)。通过比较叉角厉蝽5龄若虫、雌雄成虫触角转录组，共筛选出7 324个差异表达基因，分析发现5龄若虫与成虫间的差异表达基因较多，雌雄成虫之间差异表达基因较少；利用FPKM值对OBP与CSP基因进行表达量分析发现，大部分OBP与CSP的表达量在雌雄间差异不大，而若虫与成虫相比基因表达量差异较大，除少部分基因在5龄若虫中高表达外，其余大部分基因均在雌雄成虫间高表达。本研究获得了叉角厉蝽触角转录组数据，并鉴定出候选嗅觉相关基因，结果为进一步研究叉角厉蝽的嗅觉感受机制及昆虫化学生态奠定分子基础。

英文摘要:

Eocanthecona furcellata is a kind of natural predator which plays an important role and potential in biological control. Antennae is an important organ for insect information exchange and its olfaction-related genes are an important molecular basis for regulating insect predatory behavior. The objective of this study is to establish the antennal transcriptome database of E. furcellata. The antennal transcriptome of E. furcellata was sequenced using an Illumina platform, analyzed bioinformatically and identification and expression of olfactory related genes. In total, 67 843 unigenes with N50 length of 2 300 bp were obtained. After annotating for unigenes in gene function database, 27 686 unigenes obtained functional annotation. Information 33.33% unigenes were annotated to the NR database. E. furcellata unigenes had the highest similarity(64.2%) to those of Halyomorpha halys. 14 258 unigenes were divided into 42 branches and three categories(biological processes, cellular components and molecular function) using Gene Ontology(GO). In the KEGG database, 7 703 unigenes were assigned to 282 known metabolic pathways. Among these, 11.50% unigenes were involved in the signal transduction pathway. By further analyzing transcriptome data, 134 olfaction-related genes of G. mellonella including 10 odorant binding protein(OBP) genes, 32 chemosensory protein(CSP) genes, 156 odorant receptor(OR) genes, 48 ionotropic receptor(IR) genes and 6 sensory neuron membrane protein(SNMP) genes. By comparing the transcriptome of 5th instar nymph, female and male adults, 7 324 differentially expressed genes were identified. The results showed that there were more differentially expressed genes between 5th instar nymph and adult, but less differentially expressed genes between male and female adults. In this study, FPKM value was used to analyze the gene expression of OBP and CSP. It was found that the gene expression of OBP and CSP had no significant difference between male and female. Most of the genes were up-regulated between male and female adults except a few of them were up-regulated in 5th instar nymphs. This study acquired the antennal transcriptome data of E. furcellata and identified olfaction-related genes. The results provide a foundation for the further study of the molecular mechanisms and chemical ecology of insects of olfactory reception in E. furcellata.

查看全文查看/发表评论下载PDF阅读器

关闭