《环境昆虫学报》

自柑橘木虱Diaphorina citri体上分离虫生真菌，通过形态特征和ITS序列分析，鉴定虫生真菌的种类；通过致病性测定，明确昆虫病原真菌种类。结果发现，自死体柑橘木虱上分离到18个虫生真菌菌株，隶属4种真菌；自活体上分离到985个虫生真菌菌株，隶属25种真菌。致病性测定结果发现，仅4种真菌对柑橘木虱成虫有致病性，包括刀孢蜡蚧菌Lecanicillium psalliotae、球孢白僵菌Beauveria bassiana、爪哇虫草Cordyceps javanica和淡紫紫孢菌Purpureocillium lilacinum，该4种真菌的分生孢子悬浮液（浓度5×10^7个孢子/mL）接种柑橘木虱成虫后10 d的累计死亡率分别为100%、100%、98.89%和43.33%。其中爪哇虫草仅自死体柑橘木虱上分离到，刀孢蜡蚧菌仅在活体上分离到，球孢白僵菌和淡紫紫孢菌在死体和活体上均被分离到。可见，柑橘木虱活体上的虫生真菌种类丰富。生产上应加强保护和利用昆虫病原真菌，提高其对柑橘木虱的自然抑制力。

英文摘要:

Entomogenous fungi were isolated from Diaphorina citri and their species were identified by morphological characteristics and ITS sequence analysis; the species of entomopathogenic fungi were determined by pathogenicity assay. The results showed that 18 entomogenous fungal strains were isolated from corpse of D.citri, belonging to 4 species of fungi; 985 entomogenous fungal strains were isolated from living D.citri, belonging to 25 species of fungi. The results of pathogenicity assay showed that only 4 species of fungi were entomopathogenic fungi of D.citri, including Lecanicillium psalliotae, Beauveria bassiana, Cordyceps javanica and Purpureocillium lilacinum. The cumulative mortality of D.citri adult were 100%, 100%, 98.89% and 43.33%, 10 days after inoculated with 5×10^7 spores/mL conidia concentration of the 4 entomopathogenic fungi respectively. Among 4 entomopathogenic fungi, C.javanica was only isolated from corpse of D.citri, L.psalliotae was only isolated from living D.citri, B.bassiana and P.lilacinum were isolated from both corpse and living D.citri. It is visible that in living D.citri, there were abundant entomogenous fungi species. The entomopathogenic fungi should be strengthen in protection and utilization in citrus production, to improve the natural inhibition of D.citri.

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