| 李新宇，马卫华，杜亚丽，徐凯，姜玉锁,2022,中华蜜蜂ZFP37基因的生物信息学分析及高温下表达特性的研究[J].环境昆虫学报,(4):986-993 |
| 中华蜜蜂ZFP37基因的生物信息学分析及高温下表达特性的研究 |
| Bioinformatics analysis of ZFP37 gene and expression characteristcs in high temperature stress in Apis cerana cerana |
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| DOI： |
| 中文关键词: ZFP37 中华蜜蜂 高温胁迫 生物信息 表达模式 |
| 英文关键词:ZFP37 Apis cerana cerana heat stress bioinformatics expression characteristcs |
| 基金项目:现代农业产业技术（蜜蜂）体系建设专项（CARS-44-KXJ22) |
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| 中文摘要: |
| 锌指蛋白（Zinc finger proteins，ZFPs）是一类在真核生物体内广泛分布的蛋白质。锌指蛋白作为一类转录因子，它能够调控基因的表达和细胞的分化，最近的研究显示其在动植物抗逆方面也发挥着重要作用。本研究对中华蜜蜂 Apis cerana cerana ZFP37的蛋白结构进行了预测分析，并通过qRT-PCR分析了中华蜜蜂在遭受高温胁迫时ZFP37的表达情况，进一步了解锌指蛋白在中华蜜蜂应对热胁迫过程中的作用。结果显示，中华蜜蜂ZFP37可编码123个氨基酸，蛋白分子量为13.7 kDa，无跨膜结构。氨基酸同源序列比对结果表明，中华蜜蜂ZFP37序列与蜜蜂科昆虫的相似性最高，与其他膜翅目昆虫的相似性存在差异。基因的表达模式显示，ZFP37在高温下表达升高，此外，胁迫时间的增加也可导致ZFP37表达的升高。这些结果表明ZFP37对于中华蜜蜂应对热应激有重要的生物学意义。 |
| 英文摘要: |
| ZFPs (zinc finger protein) are a class of proteins widely distributed in eukaryotic organisms. As a type of transcription factor, ZFPs can regulate gene expression and cell differentiation. Recent studies had shown that it also played an important role in the resistance of animals and plants. In this study, the protein structure of Apis cerana cerana ZFP37 was predicted and analyzed, and the expression of ZFP37 of the A. cerana cerana under heat stress was analyzed by qRT-PCR to further understand the zinc finger protein in the A. cerana cerana in response to heat the role of stress in the process. The results showed that the AcerZFP37 could encode 123 amino acids, with a protein molecular weight of 13.7 kDa, and no transmembrane structure. The result of amino acid homologous sequence alignment showed that the AcerZFP37 sequence had the highest similarity with Apidae insects, and there were differences in similarity with other Hymenoptera insects. The gene expression pattern showed that the expression of ZFP37 increases at high temperature. In addition, the increase of stress time could also lead to the increase of ZFP37 expression. These results indicated that ZFP37 had important biological significance for A. cerana cerana to cope with heat stress. |
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