| 覃英灿，容万韬，李月妹，陆桂红，李晓东,2022,眼优角蚱两性成体转录组差异分析[J].环境昆虫学报,(4):968-977 |
| 眼优角蚱两性成体转录组差异分析 |
| Differential analysis of female and male transcriptome in Eucriotettix oculatus |
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| DOI： |
| 中文关键词: 眼优角蚱 转录组 雌雄差异 昆虫 |
| 英文关键词:Eucriotettix oculatus transcriptome sexual dimorphism insect |
| 基金项目:国家自然科学基金（31702049）；广西高等学校千名中青年骨干教师培育计划（201912）；广西自然科学基金（2020GXNSFAA159116）；广西壮族自治区大学生创新创业训练计划项目（S202110605084） |
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| 中文摘要: |
| 为了解蚱类昆虫两性间基因转录水平上的整体差异，本研究以眼优角蚱Eucriotettix oculatus为研究对象，通过高通量转录组（RNA-Seq）测序和分析技术对其雌、雄成体的转录组进行了测定和分析。结果显示，在雌、雄转录组中共获得13 432条转录本，两性间差异表达基因共3 597个，其中雌性相对于雄性显著上调基因1 295个，显著下调基因2 302个。对其中9个差异基因进行实时荧光定量PCR验证，其结果与RNA-Seq分析一致。差异基因显著富集的GO条目大多是与细胞的形成有关，结合、细胞组分和细胞过程等条目相关基因显著高表达。KEGG富集显示差异基因主要是与性腺发育、遗传信息加工、能量代谢和消化等通路有关。研究结果对从分子水平进一步研究眼优角蚱两性差异提供一定的理论基础。 |
| 英文摘要: |
| In order to understand the overall differences in gene transcription between the sexes of pygmy grasshoppers, Eucriotettix oculatus was determined and analyzed by high-throughput transcription group (RNA-Seq) sequencing and analysis techniques. The results showed that 13 432 transcriptions were obtained in females and males, and a total of 3 597 genes were differentially expressed between the sexes, of which 1 295 genes were significantly up-regulated and 2 302 genes were significantly down regulated in females compared with males. Real-time fluorescence quantitative PCR validation for nine of the different genes was consistent with RNA-Seq analysis. Most of the GO entries with significantly enrichment of differential genes were related to cell formation, and the genes related to binding, cellular component and cellular processes were significantly overexpressed. KEGG enrichment showed that differential genes were mainly related to pathways such as gonad development, genetic information processing, energy metabolism, and digestion. The results provided a theoretical basis for further study on the gender differences of E. oculatus at the molecular level. |
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