刘伟娇,张肖丽,高雪珂,朱香镇,王丽,姬继超,雒珺瑜,崔金杰,李东阳,张开心,2022,5个棉蚜组织蛋白酶B基因克隆及在不同寄主专化型中的表达分析[J].环境昆虫学报,(4):946-955
5个棉蚜组织蛋白酶B基因克隆及在不同寄主专化型中的表达分析
Clone and expression analysis of five cathepsin B genes in different host-specific types of Aphis gossypii Glover
  
DOI:
中文关键词:  棉蚜  组织蛋白酶B  发育阶段  寄主专化型  表达谱
英文关键词:Aphis gossypii  Cathepsin B  developmental stage  host specialization  expression profile
基金项目:中国农业科学院科技创新工程
作者单位
刘伟娇,张肖丽,高雪珂,朱香镇,王丽,姬继超,雒珺瑜,崔金杰,李东阳,张开心 中国农业科学院棉花研究所棉花生物学国家重点实验室河南安阳 455000 
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中文摘要:
      昆虫组织蛋白酶B在昆虫代谢中发挥着重要作用。本研究从棉蚜Aphis gossypii Glover的转录组数据库中挑选Cath B并克隆得到全长cDNA序列,使用qRT-PCR分析这些基因在不同寄主专化型棉蚜不同龄期中的相对表达量。通过分析棉蚜体内5个高表达组织蛋白酶B基因不同发育阶段的表达谱,比较了不同专化型棉蚜在寄主转换之后的表达差异。研究发现,这5个基因在不同龄期不同寄主上均表达。棉花型棉蚜在棉花寄主上不同基因间的表达各不相同。黄瓜型棉蚜在黄瓜Cucumis sativus L.上AgCb-1、AgCb-2、AgCb-3基因均在成蚜时期表达量相对较高,AgCb-4、AgCb-5基因表达量则与之相反。分别将棉花型棉蚜和黄瓜型棉蚜转接到西葫芦Cucurbita pepo L.上后,棉花型棉蚜AgCb-1、AgCb-4基因和黄瓜型棉蚜AgCb-1、AgCb-2、AgCb-3基因均在成蚜不同时期的表达量显著大于转接前。结果表明:AgCb-1、AgCb-2、AgCb-3、AgCb-4、AgCb-5基因表达量伴随着棉蚜的生长发育以及寄主的转换,表现出不同的差异。说明组织蛋白酶在棉蚜对寄主植物的适应性过程中可能发挥作用。
英文摘要:
      Cathepsin B plays an important role in insect metabolism. In this study, Cathepsin B was selected from the transcriptome database of Aphis gossypii Glover and cloned into full-length cDNA sequences. The relative expression levels of these genes in different host specific types of A. gossypii at different instars were analyzed by qRT-PCR. The expression profiles of five highly expressed Cathepsin B genes in A. gossypii at different developmental stages were analyzed to compare the expression of different specific types of A. gossypii after host conversion. All the five genes were expressed in different instars and different hosts. The expression of different genes in cotton host was different. The expression levels of AgCb-1, AgCb-2 and AgCb-3 in the cucumber type were relatively high in the adult stage, while the expression levels of AgCb-4 and AgCb-5 were opposite. A. gossypii of cotton type and cucumber type were transferred to Cucurbita pepo L., respectively. The expression levels of AgCb-1 and AgCb-4 genes of cotton type and AgCb-1, AgCb-2 and AgCb-3 genes of cucumber type were significantly higher in different periods than before transfer. The results showed that the expression levels of AgCb-1, AgCb-2, AgCb-3, AgCb-4 and AgCb-5 were different with the growth and development of A. gossypii and the conversion of host plants. This suggested that Cath B may play a role in the adaptation of cotton aphid to host plants.
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