钱正明,周妙霞,方琼谜,温宝庆,李文佳,吕根品,李文庆,2022,基于核壳型亲水作用色谱技术同时测定冬虫夏草中核苷和糖醇类成分的含量[J].环境昆虫学报,(2):491-498
基于核壳型亲水作用色谱技术同时测定冬虫夏草中核苷和糖醇类成分的含量
Simultaneous determination of nucleosides and sugar alcohols in Ophiocordyceps sinensis by core-shell hydrophilic interaction liquid chromatography
  
DOI:
中文关键词:  冬虫夏草  核苷  糖醇  核壳型亲水作用色谱  同时测定
英文关键词:Ophiocordyceps sinensis  nucleoside  sugar alcohol  core-shell hydrophilic interaction liquid  simultaneous determination
基金项目:国家重点研发计划(2018YFC1706101)
作者单位
钱正明,周妙霞,方琼谜,温宝庆,李文佳,吕根品,李文庆 1.暨南大学药学院广州 5106322.乳源东阳光药业有限公司广东韶关 5127213.东莞市东阳光冬虫夏草研发有限公司广东东莞 523850 
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中文摘要:
      核苷和糖醇类成分是冬虫夏草Ophiocordyceps sinensis中两类重要的活性成分,常被作为冬虫夏草质量的评价指标。本研究采用核壳型亲水作用色谱柱技术,建立同时测定冬虫夏草中核苷和糖醇类成分含量的快速液相色谱分析方法。结果表明,在8 min内尿苷、腺苷、肌苷、鸟苷和甘露醇均实现基线分离,且各成分线性关系良好,日内精密度、日间精密度和重复性均小于3%,平均回收率在99.0%~106.1%,供试品溶液在24 h内稳定。20批冬虫夏草的尿苷、腺苷、肌苷、鸟苷和甘露醇含量与文献报道结果基本一致。本研究建立的方法快速、准确、可靠,可用于冬虫夏草核苷和糖醇类成分的同时测定。
英文摘要:
      Nucleosides and sugar alcohols, two important active components in Ophiocordyceps sinensis, were often employed as quality control markers. In this study, a rapid HPLC method for simultaneous determination of nucleosides and sugar alcohols in O. sinensis by core-shell hydrophilic interaction liquid chromatography(HILIC)was developed. The results showed that uridine, adenosine, inosine, guanosine and mannitol were well separated in 8 min. The calibration curves of the investigated compounds showed good linearity. The RSD of precision, repeatability and stability were all less than 3%. The average recoveries ranged from 99.0% to 106.1%. Compared with previous reports, the contents of uridine, adenosine, inosine, guanosine and mannitol in O.sinensis samples were consistent. The developed method was rapid, accurate and reliable for the simultaneous determination of nucleosides and sugar alcohols in O.sinensis.
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