寇若玫,窦飞越,李红英,周泽扬,龙金桂,黄敦元,2022,油茶地蜂不同龄期幼虫肠道细菌多样性及其差异分析[J].环境昆虫学报,(2):402-413
油茶地蜂不同龄期幼虫肠道细菌多样性及其差异分析
Gut bacterial divergence between different larval instars of Andrena camellia Wu (Hymenoptera: Andrenidae)
  
DOI:
中文关键词:  油茶地蜂  幼虫  高通量测序  肠道细菌  细菌多样性
英文关键词:Andrena camellia Wu  larva  high-throughput sequencing  gut bacteria  bacterial diversity
基金项目:科技部科技基础资源调查专项(2018FY100405);国家自然科学基金(31970484,31770160);成渝地区双城经济圈建设科技创新项目上(kjcx2020021)
作者单位
寇若玫,窦飞越,李红英,周泽扬,龙金桂,黄敦元 重庆师范大学媒介昆虫重庆市重点实验室重庆 401331 
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中文摘要:
      蜜蜂肠道微生物在其宿主健康与调控宿主生长发育中起着重要作用。油茶地蜂Andrena camellia Wu是油茶的优势传粉昆虫。本研究从野外挖掘该蜂典型样地的巢穴得到油茶地蜂1~5龄及滞育阶段的幼虫,通过PCR扩增得到幼虫肠道微生物16S rRNA基因文库,利用Illumina Miseq二代高通量测序技术首次研究了油茶地蜂不同龄期幼虫肠道细菌的群落结构和多样性。结果显示,油茶地蜂幼虫肠道细菌菌群共检测到27个门,72个纲,155个目,278个科,570个属。其中,最主要的门为变形菌门Proteobacteria(占40.05%),最主要的目为乳杆菌目Lactobacillales(占12.16%),最主要的科为乳杆菌科Lactobacillaceae(占11.27%),最主要的属是乳杆菌属Lactobacillus(占11.27%)。核心细菌群落组间差异、Alpha多样性以及Beta多样性分析显示:不同龄期油茶地蜂幼虫肠道细菌群落在组成、多样性以及结构上均存在显著差异。线性判别分析结果显示,不动杆菌属Acinetobacter和红球菌属Rhodococcus主要存在于5龄幼虫阶段,巴尔通氏体属Bartonella和肠杆菌属Enterobacter主要存在于1龄幼虫阶段。本研究明确了油茶地蜂不同龄期幼虫肠道微生物组成、优势菌群及其群落变化规律,为油茶地蜂等土壤筑巢独栖野生蜜蜂肠道微生物学及其保护生物学的相关研究提供理论依据及研究角度。
英文摘要:
      Gut microbes play an important role in health and control of growth and development of honey bees. Andrena camellia Wu is the main solitary pollinator of Camellia oleifera. The typical nests of this bee were excavated in the wild to obtain the larvae at the 1~5th instar and diapause stage. The gene library of 16S rRNA was obtained by PCR from the DNA extracted from the gut contents of the larvae. The structure and biodiversity of gut bacterial community were studied for the first time by Illumina Miseq second-generation high-throughput sequencing technology. The results showed that 27 phyla, 72 classes, 155 orders, 278 families and 570 genera were detected in the gut flora of the larvae of A.camellia. The main phylum, order, family and genera were Proteobacteria (40.05%), Lactobacillales (12.16%), Lactobacillaceae (11.27%), and Lactobacillus (11.27%), respectively. There was significant statistical difference in core bacterial communities’ composition, biodiversity and community structure. The line discriminant analysis showed that Acinetobacter and Rhodococcus were mainly in the fifth instar larvae, while the Bartonella and Enterobacter were mainly in the first instar larvae. In this study, the composition of intestinal bacterial communities, dominant flora and community variation of different instar larvae of A. camellia were determined, which provided theoretical basis and research perspective for intestinal microbiology and protective biology of soil-nesting wild bees such as A. camellia.
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