| 舒本水，戴静华，余竟成，杨仙妹，林进添,2022,喜树碱对草地贪夜蛾幼虫中肠细菌群落组成及多样性的影响[J].环境昆虫学报,44(1):44-51 |
| 喜树碱对草地贪夜蛾幼虫中肠细菌群落组成及多样性的影响 |
| Effects of camptothecin on the composition and diversity of the bacterial community in the midgut of Spodoptera frugiperda larvae |
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| DOI： |
| 中文关键词: 草地贪夜蛾 喜树碱 中肠 细菌群落 多样性 |
| 英文关键词:Spodoptera frugiperda camptothecin midgut bacterial community diversity |
| 基金项目:广东省重点领域研发计划（2020B020223004）；广东省教育厅创新团队项目（2017KCXTD018）；大学生创新创业训练计划（202011347001）；仲恺农业工程学院本科教学质量与教学改革工程项目（KA200190261） |
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| 中文摘要: |
| 草地贪夜蛾 Spodoptera frugiperda (J.E.Smith)是一种重要的入侵害虫，对多种作物生产造成严重的威胁。喜树碱是一种单萜类吲哚生物碱，对草地贪夜蛾幼虫生长发育具有显著抑制效果。本研究采用饲料混药法将喜树碱以1 mg/kg浓度处理草地贪夜蛾4龄幼虫3 d，解剖幼虫收集中肠内容物后采用16S rDNA测序技术分析喜树碱处理对草地贪夜蛾幼虫中肠细菌群落的影响。研究结果表明，从草地贪夜蛾幼虫中肠内容物中共得到295 028条有效reads，聚类为262个OTUs，注释到17个门35个纲80个目123个科191个属246种细菌。其中优势细菌门为厚壁菌门Firmicutes和变形菌门Proteobacteria。对幼虫中肠细菌群落组成分析发现喜树碱处理后厚壁菌门Firmicutes丰度显著下降，而变形菌门Proteobacteria丰度显著上升。进一步分析发现，有79种和50种细菌为对照组和喜树碱处理组幼虫中肠特有物种。Alpha和Beta多样性分析表明，喜树碱显著改变了草地贪夜蛾幼虫中肠细菌群落多样性。本研究为丰富草地贪夜蛾幼虫肠道微生物群落数据信息及探究喜树碱对草地贪夜蛾的作用机制提供一定的理论依据。 |
| 英文摘要: |
| Spodoptera frugiperda (J.E.Smith) is an important invasive pest that poses a serious threat to the production of many crops in China. Camptothecin is a monoterpene indole alkaloid that has a significant effect on the growth and development of S. frugiperda larvae. In this study, camptothecin was fed to the fourth instar larvae with a concentration of 1 mg/kg for 3 d, then the midgut contents were collected and 16S rDNA sequencing technology was used to analyze the effect of camptothecin treatment on the midgut bacterial community. Results showed that a total of 295 028 effective reads were obtained by sequencing, clustered into 262 OTUs, and annotated into 17 phyla, 35 classes, 80 orders, 123 families, 191 genera, and 246 species. The dominant bacterial phyla were Firmicutes and Proteobacteria in all the samples. Bacterial community composition analysis indicated that the abundance of Firmicutes and Proteobacteria in the midgut changed significantly after camptothecin treatment. Further analysis revealed that 79 and 50 unique bacteria species were identified in the midguts of control and camptothecin-treated groups, respectively. Alpha and Beta diversity analysis showed that camptothecin significantly changed the bacterial community diversity of the larval midgut. This study provides a theoretical basis for enriching the data and information on the intestinal microbial community of S. frugiperda larvae and exploring the mechanism of camptothecin against S. frugiperda. |
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