| 殷先元，蒲宇辰，黄颖，黄斌，侯有明,2021,苏云金芽胞杆菌HA和HD对黄曲条跳甲成虫的毒力分析[J].环境昆虫学报,(5):1325-1330 |
| 苏云金芽胞杆菌HA和HD对黄曲条跳甲成虫的毒力分析 |
| Toxicity analysis of Bacillus thuringiensis HA and HD against Phyllotreta striolata adults |
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| DOI： |
| 中文关键词: 黄曲条跳甲 苏云金芽胞杆菌 成虫 毒力 组织病理学 |
| 英文关键词:Phyllotreta striolata Bacillus thuringiensis adults toxicity histopathological analysis |
| 基金项目:国家重点研发计划（2018YFD0201209-5）；福建省高等学校创新能力提升计划（K80ND800102B） |
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| 中文摘要: |
| 黄曲条跳甲Phyllotreta striolata (Fabricius)是为害十字花科蔬菜的世界性害虫之一。为了探究苏云金芽胞杆菌HA和HD对黄曲条跳甲成虫的防治潜力，本研究测定了苏云金芽胞杆菌HA和HD菌株对黄曲条跳甲成虫的毒力，以及对其谷胱甘肽-S-转移酶（GST）活性和中肠组织形态的影响。结果表明：Bt-HA和Bt-HD均对黄曲条跳甲成虫有杀虫活性，LC50分别为4.01×10^8 CFU/mL和1.17×10^8 CFU/mL。菌株Bt-HD处理组的GST酶活性高于对照组和菌株Bt-HA处理组。菌株Bt-HA处理黄曲条跳甲成虫14 d后，中肠微绒毛肿胀脱落；菌株Bt-HD处理黄曲条跳甲成虫4 d后中肠微绒毛开始疏松脱落，14 d后中肠柱状细胞底膜变形脱落。综上所述，苏云金芽胞杆菌HD对黄曲条跳甲成虫的毒力更强，是防治黄曲条跳甲成虫的优势菌株，其杀虫机理值得深入研究。 |
| 英文摘要: |
| The Striped flea beetle, Phyllotreta striolata (Fabricius), is one of the most destructive pests of cruciferous vegetables worldwide. To explore prevention potential of Bacillus thuringiensis HA and HD on P.striolata adults, bioassays, glutathione S-transferase (GST) enzyme activity and histopathological analysis of midgut epithelium of P. striolata adults were conducted. The results showed that the LC50 values of Bt-HA and Bt-HD were 4.01×10^8 CFU/mL and 1.17×10^8 CFU/mL respectively. GST enzyme activity in the Bt-HD treatment group was higher than that in the control group and the Bt-HA treatment group. Histopathological analysis revealed the microvilli swelled and fell off after 14 days of treatment with Bt-HA, the microvilli began to loosen and fall off after 4 days of treatment with strain Bt-HD, and the basement membrane of columnar cells in the midgut began to deform and fall off after 14 days. In conclusion, Bt-HD is more virulent to adults of P. striolata than Bt-HA. The insecticidal mechanism of B. thuringiensis HD on P. striolata adults should be further investigated. |
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