| 杨海燕，胡凯平，宋水林，王广利，徐业，王建国，李小珍,2021,橘小实蝇雌雄成虫生殖系统中可培养细菌的分离鉴定[J].环境昆虫学报,(5):1250-1256 |
| 橘小实蝇雌雄成虫生殖系统中可培养细菌的分离鉴定 |
| Isolation and identification of cultivable bacteria in the female and male adult reproductive system of Bactrocera dorsalis (Diptera: Tephritidae) |
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| DOI： |
| 中文关键词: 赣南脐橙基地 橘小实蝇 生殖系统 可培养细菌 差异 |
| 英文关键词:Gannan navel orange base Bactrocera dorsalis (Hendel) reproductive system cultivable bacteria differentiation |
| 基金项目:国家自然科学基金(31560516)；江西省教育厅科学技术研究项目(9232307675) |
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| 中文摘要: |
| 本研究分离鉴定了来自赣南脐橙基地橘小实蝇Bactrocera dorsalis (Hendel)雌性和雄性成虫生殖系统中的可培养细菌。采用LB培养基分离纯化和菌株16S rRNA基因序列分析相结合的方法，从饲养10 d的橘小实蝇雌雄成虫生殖系统，获得15株具有不同表观特征的细菌，分属变形菌门Proteobacteria、拟杆菌门Bacteroidetes、放线菌门Actinobacteria和厚壁菌门Firmicutes。橘小实蝇雌雄成虫生殖系统共同拥有4个相同属，分别是不动杆菌属Acinetobacter、苍白杆菌属Ochrobactrum、金黄杆菌属Chryseobacterium和芽孢杆菌属Bacillus。雌性成虫特有无色杆菌属Achromobacter、剑菌属Ensifer、鞘脂杆菌属Sphingobacterium、微杆菌属Microbacterium和细球菌属Micrococcus；雄性成虫特有假单胞菌属Pseudomonas和拟杆菌属Bacteroides。其中，不动杆菌属菌株数量最多，分别占雌、雄成虫生殖系统菌株数量的35.00%和31.58%，为橘小实蝇生殖系统可培养的优势细菌属。橘小实蝇雌性和雄性成虫生殖系统的内生细菌存在差异，为研究橘小实蝇性别与内生细菌的协同进化提供信息，也为分析内生细菌在橘小实蝇生殖系统中的功能奠定基础。 |
| 英文摘要: |
| Our goal was to isolate and identify cultivable bacteria in the female and male adult reproductive system of Bactrocera dorsalis (Hendel) from Gannan navel orange base. Adopting LB culture medium and bacterial strain 16S rRNA sequencing technique, 15 strains of cultivable bacteria were isolated and identified from the reproductive system of B. dorsalis adults reared under laboratory conditions for 10 days. All bacteria strains belonged to the 4 phyla Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. Both female and male adult reproductive system of B. dorsalis harbored four genera Acinetobacter, Ochrobactrum, Chryseobacterium and Bacillus. Five unique bacterial genera Achromobacter, Ensifer, Sphingobacterium, Microbacterium and Micrococcus were found in the female adult reproductive system, and two unique bacterial genera Pseudomonas and Bacteroides were discovered in the male adult reproductive system. Among them, Acinetobacter was dominated in the adult reproductive system of B. dorsalis, 35.00% and 31.58% for female and male, respectively. Cultivable bacteria in the female and male adult reproductive system of B. dorsalis were different, which provided information for exploring the coevolution between B. dorsalis gender and endophytic bacteria, and analyzing the function of those bacteria in the adult reproductive system of B. dorsalis. |
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