| 闫 艳，程梦迪，曹春桥，李 虎,2021,访花昆虫野蚜蝇线粒体基因组结构分析[J].环境昆虫学报,(4):992-1002 |
| 访花昆虫野蚜蝇线粒体基因组结构分析 |
| Structural analysis of complete mitochondrial genome of Syrphus torvus ( Osten-Sacken) of a flower-visiting insect |
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| DOI： |
| 中文关键词: 蚜蝇 线粒体基因组 系统发育 |
| 英文关键词:Syrphidae mitogenome phylogeny |
| 基金项目:陕西省科技厅自然科学基础研究计划 (2020JQ-869); 陕西理工大学开放课题研究基金 (SLGPT2019KF03-01); 陕西理工大学研究生创新基金项目 ( SLGYCX2015); 中国博士后科学基金 (2016M590968) |
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| 中文摘要: |
| 野蚜蝇 Syrphus torvus ( Osten-Sacken) 成虫具访花习性，是重要的传粉昆虫; 幼虫捕食蚜虫，是蚜虫重要天敌之一。本研究通过高通量测序、组装拼接获得了完整的野蚜蝇线粒体基因组全序列 ( GenBank 登录号: MW074962) ，其序列全长为 16 444 bp，包含 13 个蛋白编码基因 ( PCGs)、22 个 tRNAs、2 个 rRNAs 和 D-loop 区。 野蚜蝇线粒体全序列 A + T 含量为 80.6% ，G + C 含量为 19.4% ，表现出明显的 A + T 偏斜。除了 COXI、ATP8、ATP6 和 ND1 的起始密码子为 TTG，其它 9 个 PCGs 以 ATN 为起始密码子; COXI 的终止密码子为不完整的 T - -， ATP6 为 TAG，其余 11 个 PCGs 的终止子为 TAA。22 个 tRNAs 的二级结构均为典型的三叶草结构，并预测了野蚜 蝇 rRNAs 的二级结构。野蚜蝇 D-loop 区存在重复序列和茎环结构，22 种蚜蝇科基因间隔区与重叠区具有 6 个保守区域。基于 24 个物种 ( 22 个蚜蝇科和 2 个缟蝇科) 的 PCGs 序列，通过贝叶斯法 ( BI) 和最大似然法 ( ML) 构 建系统发育树，结果显示，野蚜蝇和黑足蚜蝇 Syrphus vitripennis 互为姊妹种，支持了蚜蝇亚科 Syrphinae 的单系性，两种拓扑结构中管蚜蝇亚科 Eristalinae 均没有聚为一支。该研究结果丰富了蚜蝇科线粒体基因组学基本数据，为进一步深化蚜蝇科系统发育关系讨论提供了参考。 |
| 英文摘要: |
| Syrphus torvus ( Osten-Sacken) belongs to the family Syrphidae of Diptera. The adults are flower visitors，are important pollinating insects; the larvae feed on aphids，are one of the important natural enemies of aphids. In this study，the genome of S. torvus was obtained by high-throughput sequencing and the complete mitochondrial genome ( mitogenome) was assembed ( GenBank accession No. MW074962) ． The length of complete mitogenome was 16 444 bp，including 13 protein coding genes ( PCGs) ，22 tRNAs，2 rRNAs and D-loop. There were 80.6% A + T content and 19.4% G + C content in this complete mitogenome of S. torvus，showed significant A + T bias. All of PCGs，except COXI， ATP8，ATP6，and ND1 start codons were TTG，all start codon were ATN; all stop codons were TAA but COXI was incomplete T- - and ATP6 was TAG. All tRNAs were the typical cloverleaf secondary structure. The rRNAs secondary structures of S. torvus were predicted. There were four repeats and three stem-loop structures in the D-loop of S. torvus. Six conserved regions were possessed in Intergenic nucleotides and overlap of 22 Syrphidae. The phylogenetic tree was built by PCGs of 24 species ( 22 Syrphidae and 2 Lauxaniidae) using methods of Bayesin ( BI) and Maximum Likelihood ( ML). The results showed that S. torvus and Syrphus vitripennis were sister group，support monophyly of Syrphinae， Eristalinae not gather one clade in two topology structures. The results enriched the basic data of genomics of Syrphidae mitochondrial and provide a reference for further discussion of phylogenetic relationship of Syrphidae. |
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