余源婵,杨茂发,商胜华,刘健锋,于晓飞,2021,不同昆虫取食对烟草信号分子和防御酶的影响[J].环境昆虫学报,(4):967-977
不同昆虫取食对烟草信号分子和防御酶的影响
Effects of different insects feeding on tobacco signaling molecules and defense enzymes
  
DOI:
中文关键词:  不同昆虫  取食  烟草  信号分子  防御酶
英文关键词:Different insect  feeding  tobacco  signal molecules  defense enzymes
基金项目:中国烟草总公司贵州省公司重大科技专项 (201603,201752010040001) ; 贵州省高层次创新型人才培养项目 ([2016]-4022)
作者单位
余源婵,杨茂发,商胜华,刘健锋,于晓飞 1. 贵州大学昆虫研究所/贵州山地农业病虫害重点实验室贵阳 550025
2. 贵州大学烟草学院贵阳 550025
3. 贵州省烟草科学研究院贵阳 550081 
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中文摘要:
      以烟草 Nicotiana tabacum L. 品种 MSK326’为研究材料,采用生化分析法研究了 3 种昆虫取食对烟草防御信号分子含量和防御酶活性的影响。结果显示: 棉铃虫 Helicoverpa armigera Hübner 取食后,烟叶中的 JA、NO 含量分别在 48 h、24 h 时达到最高,为对照的 1.1 倍和 1.35 倍,斜纹夜蛾 Spodoptera litura Fabricius 和烟蚜 Myzus persicae Sulzer 取食后,烟叶中的 JA、NO 含量分别较对照降低 7.1% 、33.33% 和 10.38%、60% ; 棉铃虫和斜纹夜 蛾取食后 H2S 含量均在 6 h 达到最高,分别较对照增加 25.36% 和 73.7% ,烟蚜取食后 H2S 含量则在 48 h 时达到峰值,较对照增加 77.03% 。棉铃虫取食下烟叶内 PAL、PPO 和 POD 均呈先降低后增加的波动性变化,分别在 72 h、48 h 和 48 h 时较对照显著升高; 斜纹夜蛾取食后,烟叶内 PAL 和 POD 与棉铃虫取食存在相似变化趋势,分别在 72 h 和 24 h 时升至峰值,PPO 活性呈持续升高趋势,12 h 时达到最高; 棉铃虫或斜纹夜蛾取食后,烟叶内 LOX 活性受到抑制,分别在 72 h 和 48 h 时降至最低; 烟蚜取食后,烟叶内 PAL 活性呈先降低后增加的波动性变化,仅在 48 h 时较对照显著升高,LOX、PPO 和 POD 则均呈先升高后降低的波动趋势,分别在 6 h、6 h 和 12 h 时较对照显著升高。综上说明,昆虫取食诱导烟株激活体内生理防御机制差异与昆虫的种类、取食方式及取食时间相关,该结果为烟草生产中外源诱导增强烟草抗性及与昆虫相关的化学激发子的开发提供参考。
英文摘要:
      In this paper,tobacco “MS K326”was used as research material. The effects of feeding-modes of different insects on the signal contents and defense enzyme activities in leaves of tobacco were measured by biochemical assays. The results showed that Helicoverpa armigera feeding could induce the contents of JA,NO and H2 S reached the maximum at 48 h,24 h and 6 h,being 1. 1 ~ 1.35 times of the control group; The contents of JA and NO in tobacco leaves fed by Spodoptera litura decreased by 7.1% and 33.33%,and by 10.38% and 60% after Myzus persicae Sulzer feeding,respectively; After H. armigera and S. litura feeding,the contents of H2 S in tobacco leaves reached its highest at 6 h,and increased by 25.36% and 73.7% ,respectively. However,after M. persicae feeding,the contents of H2 S reached the maximum at 48 h,increasing by 77.03% ; After H. armigera feeding,the activities of PAL,PPO and POD decreased at first and then increased,and increased obviously at 72 h,48 h and 48 h comparing with the control; There were similar changes on activities of PAL and POD in leaves fed by S. litura: decreased at first and then increased,peaked at 72 h and 24 h,while the activity of PPO continued to raise and reached the maximum at 12 h; H. armigera and S. litura feeding caused inhibition of LOX activity,minimized at 72 h and 48 h; After M. persicae feeding,the activity of PAL did not significantly changed until it increased obviously at 48 h,and the activity of LOX,PPO and POD increased first and then decreased,which increased obviously at 6 h,6 h and 12 h,respectively. Taken together,the differences in physiological defense mechanisms of tobacco induced by insect feeding are closely related to types of pests,feeding modes and time,this result provide reference for the development of exogenous inducer to enhance tobacco resistance and develop insect-related chemical elicitors in tobacco production.
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