| 刘佰明，王芳，刘茹，杨泽众，白义川，谷希树,2021,亚致死剂量溴氰虫酰胺对MED烟粉虱取食行为及传播TYLCV的影响[J].环境昆虫学报,(3):537-544 |
| 亚致死剂量溴氰虫酰胺对MED烟粉虱取食行为及传播TYLCV的影响 |
| Effects of sublethal dose cyantraniliprole on feeding behavior and TYLCV transmission of Bemisia tabaci MED |
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| DOI： |
| 中文关键词: 溴氰虫酰胺 烟粉虱 取食行为 传毒 亚致死剂量 |
| 英文关键词:Cyantraniliprole Bemisia tabaci feeding behavior virus transmission sublethal dose |
| 基金项目:国家自然科学基金（31772171）；国家重点研发计划支持项目（2019YFD1002100）；天津市“131”创新型人才团队（201931）；天津市农业科学院青年创新与实验项目（201903） |
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| 中文摘要: |
| 取食是烟粉虱 Bemisia tabaci （Gennadius）传播植物病毒最重要的途径之一。本研究利用昆虫刺探电位（EPG）技术研究了杀虫剂溴氰虫酰胺亚致死浓度（LC15）对携带番茄黄化曲叶病毒（TYLCV）MED烟粉虱取食行为和传毒效率的影响。结果表明，溴氰虫酰胺对未携毒和携毒MED烟粉虱的亚致死浓度（LC15）分别为1.52 mg/kg 和1.75 mg/kg，差异不显著。携毒烟粉虱在溴氰虫酰胺亚致死浓度处理的番茄苗上取食，第一次刺探起始时间显著推迟，是对照番茄苗上的3.7倍。在韧皮部取食阶段，处理组烟粉虱在韧皮部分泌唾液平均时间、吸食汁液总时间和平均时间均显著缩短，分别是对照组的0.10倍、0.14倍和0.10倍，韧皮部取食能力显著降低。qPCR试验研究表明，溴氰虫酰胺亚致死浓度处理极显著的降低了烟粉虱的传毒效率，携毒烟粉虱取食48 h的番茄苗培养10 d后，TYLCV相对含量仍接近于0，表明溴氰虫酰胺对烟粉虱传播TYLCV具有良好的控制潜力。研究结果为科学合理的使用化学农药防治烟粉虱及其传播的双生病毒病提供理论依据。 |
| 英文摘要: |
| Feeding is one of the most important way for Bemisia tabaci to transmit plant virus. In this study, the effects of sublethal concentration (LC15)of cyantraniliprole on feeding behavior and virus transmission efficiency of B. tabaci MED vectored tomato yellow leaf curl virus (TYLCV)were studied using electrical penetration graph (EPG)techniques. The results indicated that the sublethal concentration (LC15)of cyantraniliprole for non-viruliferous and viruliferous B. tabaci was 1.52 mg/kg and 1.75 mg/kg, respectively. There was no significant difference between the two sublethal concentrations. When fed on tomato plants treated with LC15 of cyantraniliprole, B. tabaci start probing much more delayed, and with 3.7 multiple than on the control plants. In the phloem feeding phase, the mean and total duration of saliva secretion, mean duration of phloem sap ingestion were significant shortened, they were 0.10, 0.14 and 0.10 multiple than control groups respectively. This indicated that phloem feeding ability was significantly decreased. Experiment by qPCR showed that the LC15 of cyantraniliprole significantly reduced the transmission efficiency of B. tabaci. The relative TYLCV load in tomato plants was still close to "0" after 10 d cultivating when feeding by viruliferous B. tabaci for 48 h. This indicated that cyantraniliprole showed a perfect control potential for TYLCV. The results will provide theoretical basis to the scientific use of chemical pesticides to control B. tabaci and geminivirus disease it's transmitted. |
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