汤夏安,李贤良,池章辰,韦舒蔚,邓业成,邓志勇,骆海玉,邓明学,2021,柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应[J].环境昆虫学报,(2):485-491
柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应
Tendency response of Diaphorina citri Kuwayama to shoots and volatile components of twelve host plants
  
DOI:
中文关键词:  柑橘木虱  寄主植物  挥发物  趋性反应
英文关键词:Asian citrus psyllid  host plant  volatiles  tendency response
基金项目:广西科技重大专项(桂科AA18118046)
作者单位
汤夏安,李贤良,池章辰,韦舒蔚,邓业成,邓志勇,骆海玉,邓明学 1. 广西师范大学生命科学学院珍稀濒危动植物生态与环境保护省部共建教育部重点实验室广西 桂林 541004 2. 广西特色作物研究院广西 桂林 541004 
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中文摘要:
      为了发现对柑橘木虱 Diaphorina citri Kuwayama具有引诱活性的寄主植物嫩梢挥发物,采用Y型嗅觉仪在室内测定柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应,采用固相微萃取技术(SPME)和气-质联用分析技术(GC-MS)分析鉴定嫩梢挥发物的共同成分。结果表明,柑橘木虱对九里香 Murraya exotica 、沙田柚 Citus maxima ‘Shatian You’、红肉酸柚 Cgrandis ‘Hongrousuan You’、HB柚 Citus grandis ‘HB’、高斑柚 Citus grandis‘gaoban You’和纽荷尔脐橙Citus sinensis‘Newhall’ 6种植物嫩梢具有显著的趋性反应,选择率分别为66.70%、66.04%、63.77%、63.86%、61.67%和60.35%。从寄主植物嫩梢中鉴定出8种挥发物共同成分,分别为α-石竹烯、(-)-β-石竹烯、萜品油烯、芳樟醇、(+)-α-蒎烯、3-蒈烯、D-柠檬烯和γ-松油烯。柑橘木虱对8种植物挥发物成分均有一定的趋性,其中对浓度为10 μL/mL (-)-β-石竹烯和D-柠檬烯的选择率分别为73.20%和71.88%,对浓度为1 μL/mL芳樟醇的选择率为79.19%。研究结果为进一步开发柑橘木虱引诱剂奠定了基础。
英文摘要:
      In order to find the volatile components of shoots of the host plants with attractive activity to citrus psyllid Diaphorina citri Kuwayama, Y-type olfactory instrument was used to measure the tendency response of citrus psyllid to the shoots of twelve host plants and their volatile components, and solid phase microextraction (SPME)and gas-mass spectrometry (GC-MS)analysis were used to identify the common components of volatiles of the shoots. The results showed that the citrus psyllid had significant selective response to the shoots of Murraya exotica , Citrus maxima‘Shatian You’, Citrus grandis ‘Hongrousuan You’, Citrus grandis ‘HB’, Citrus grandis‘gaoban You’and Citrus sinensis ‘Newhall’, and the selective rates were 66.70%, 66.04%, 63.77%, 63.86%, 61.67% and 60.35%, respectively. Eight common volatile constituents were identified from the shoots volatiles of host plants, namely α-humulene, (-)-β-caryophyllene, terpinolene, linalool, ( + )-α-pinene, 3-carene, D-limonene and γ -terpinene. Citrus psyllid has selective response to the 8 plant volatile components. The selective rates to (-)-β-caryophyllene and D-limonene at 10 μL/mL reach 73.2% and 71.88% respectively, and the selective rate to linalool at 1 μL/mL reach 79.19%. The results laid the foundation for further development of attractants of citrus psyllid.
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