| 宁忠雄，李维政，梁赫，秦子昕，王冬梅，李海强，刘冰，路伟,2021,南疆三地棉铃虫田间种群对Bt棉的抗性频率监测[J].环境昆虫学报,(2):479-484 |
| 南疆三地棉铃虫田间种群对Bt棉的抗性频率监测 |
| Frequency of resistance to Bt cotton of the field population of Helicoverpa armigera in Southern Xinjiang |
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| DOI： |
| 中文关键词: Bt棉 棉铃虫 Cry1Ac蛋白 抗性基因频率 南疆 |
| 英文关键词:Helicoverpa armigera Bt cotton Cry1Ac toxin protein resistance gene frequency Southern Xinjiang |
| 基金项目:国家自然科学基金(31660520)；国家重点研发计划(2017YFD0201903)；新疆农科院科技创新重点培育专项(xjkcpy-2020004) |
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| 中文摘要: |
| 为了监测南疆主要Bt棉区棉铃虫田间种群对Bt棉的抗性频率，分别采集库尔勒、阿克苏、泽普三地的棉铃虫单雌系，以Bt毒蛋白作为人工饲料，采用单雌系F1代法进行棉铃虫田间种群抗性个体检测。本文从库尔勒、阿克苏、泽普三地分别筛选了57个、106个、92个棉铃虫单雌系。三地棉铃虫单雌系幼虫在正常饲料和Cry1Ac饲料上的平均发育级别呈线性相关，相对平均发育级别平均值分别为0.5210、0.4935、0.4623，无≥0.8的个体，估测南疆三地棉铃虫种群的Bt抗性基因频率均小于0.001。泽普玉米种植比例较高，可有效稀释棉铃虫种群的Bt抗性基因，因此泽普的棉铃虫种群敏感度最高。本研究可为新疆Bt棉区棉铃虫的抗性治理提供科学依据。 |
| 英文摘要: |
| In order to monitor the resistance frequency of the field population of Helicoverpa armigera to Bt cotton in Bt cotton areas of Southern Xinjiang, the single female lines of H. armigera were collected from Korla, Akesu and Zepu and Bt toxin protein was used as artificial feed. In this paper, 57, 106 and 92 monofemale lines of H.armigera were screened from Korla, Akesu and Zepu, respectively. There was a linear correlation between the average developmental levels of monofemale larvae of H. armigera in normal diet and Cry1Ac diet. The average values of relative developmental levels were 0.5210, 0.4935 and 0.4623, respectively.There were no individuals ≥ 0.8, which indicating that the sensitivity of H. armigera population in Zepu was the highest, and the frequency of Bt resistance gene was less than 0.001. Zepu corn planting proportion is higher, which can effectively dilute the Bt resistance gene of H.armigera population. Therefore, Zepu cotton bollworm population has the highest sensitivity.This study can provide a scientific basis for the resistance management of H. armigera in Xinjiang Bt cotton planting area. |
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