| 刘苏，蒋秀云，陈诚，汪正威，蒋兴川,2021,大蜡螟成虫3个醛氧化酶基因的鉴定、序列特征与组织表达模式[J].环境昆虫学报,(2):397-405 |
| 大蜡螟成虫3个醛氧化酶基因的鉴定、序列特征与组织表达模式 |
| Identification, sequence characteristics, and tissue expression patterns of three aldehyde oxidase genes in the adults of the greater wax moth Galleria mellonella |
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| DOI： |
| 中文关键词: 大蜡螟 醛氧化酶 系统进化分析 基因组分布 表达谱 |
| 英文关键词:Galleria mellonella aldehyde oxidases phylogenetic analysis genomic location expression profiles |
| 基金项目:国家重点研发计划（2017YFD0200902）；国家自然科学基金（31801806）；安徽省高等学校自然科学研究项目（KJ2017A868） |
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| 中文摘要: |
| 醛氧化酶（AOXs）在昆虫的嗅觉生理代谢过程中起重要作用。本研究从大蜡螟Galleria mellonella 成虫中鉴定了3个 AOXs基因，命名为 GmelAOX1、GmelAOX2 和 GmelAOX3 。这3个基因均含有完整的开放阅读框，所编码的蛋白质均具有醛氧化酶的典型特征，如具有铁硫氧化还原中心、黄素腺嘌呤二核苷酸结合区域和钼辅因子结合区域。系统进化分析显示3个 GmelAOXs被聚在不同的进化分支，且 GmelAOXs与鳞翅目AOXs亲缘关系最近。GmelAOX1 和 GmelAOX3 位于同一个基因组框架（scaffold69）上，而 GmelAOX2 位于scaffold96。 GmelAOX1 、 GmelAOX2 和 GmelAOX3 的外显子数量分别为17个、16个和21个。 GmelAOX2 高量表达于成虫触角，且在触角中的表达量显著高于其它组织，推测GmelAOX2 可能编码一个气味降解酶并参与醛类气味分子的降解。 GmelAOX1和 GmelAOX3 的表达没有组织特异性，二者编码的蛋白可能兼具气味降解和外源醛类代谢的功能。 |
| 英文摘要: |
| In insects, aldehyde oxidases (AOXs)play a critical role in the olfactory sensation and biotransformation process. In this study, three AOX genes were identified from the adults of the greater wax moth, Galleria mellonella, and named as GmelAOX1, GmelAOX2 and GmelAOX3 , respectively. All of the three genes contained complete open reading frames, and their corresponding coding proteins all have the typical features of AOX , including the iron sulfur redox centers, flavin adenine dinucleotide (FAD)-binding domain and MoCo cofactor binding domain. Phylogenetic analysis showed that three GmelAOXs were separated into different clusters, and that these GmelAOXs were closely related to their Lepidopteran orthologs. Both of GmelAOX1 and GmelAOX3 were located in the same genomic scaffold (scaffold69), whereas GmelAOX2 was in scaffold96. The number of exons in GmelAOX1 , GmelAOX2 and GmelAOX3 were 17, 16 and 21, respectively.GmelAOX2 was highly expressed in the antennae of adults, and the transcription level of this gene in antennae was significantly higher than those in other tissues, suggesting that GmelAOX2 might encode an odorant degrading enzyme involved in the aldehyde degradation. The expression of GmelAOX1 and GmelAOX3 was not tissue-specific,implying that proteins encoded by the two genes might be involved in the degradation of odorants as well as metabolism of exogenous aldehydes. |
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