| 徐天梅，黄钰，肖关丽，陈斌,2021,白背飞虱雌雄成虫肠道可培养细菌种类组成及差异分析[J].环境昆虫学报,(1):158-169 |
| 白背飞虱雌雄成虫肠道可培养细菌种类组成及差异分析 |
| Composition and differential analysis of culturable bacteria community in the gut of male and female Sogatella furcifera（Horváth） |
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| DOI： |
| 中文关键词: 白背飞虱 雌雄成虫 肠道细菌 分离鉴定 |
| 英文关键词:Sogatella furcifera（Horváth） male and female adults intestinal bacteria isolation and identification |
| 基金项目:省部共建云南生物资源保护与利用国家重点实验室开放课题(gzkf201901)；国家自然科学基金(31660537)；云南农业大学学生创新基金(2020ZKX090) |
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| 中文摘要: |
| 为弄清白背飞虱成虫肠道细菌组成及雌雄成虫之间的组成差异，本研究采用传统培养法，对云南省元江县水稻田白背飞虱雌雄成虫肠道可培养细菌种类组成进行了分离培养，并采用形态及16S rDNA序列比对方法进行细菌的种属鉴定。结果表明：从白背飞虱肠道内分离鉴定出细菌3门8科12属20种，优势菌门均为厚壁菌门Firmicides（50%），雌成虫肠道优势菌属为葡萄球菌属Staphylococcus（25%），优势菌种为松鼠葡萄球菌Staphylococcus squirrel（25%）；雄成虫肠道优势菌属为芽孢杆菌属Bacillus（22%）和微杆菌属Microbacter（22%），优势菌种为产左聚糖微杆菌Microbacterium laevaniforman（22%）。其中，雌性成虫肠道内共分离获得细菌3门8科8属12种，包括芽孢杆菌属Bacillus 3种；葡萄球菌属Staphylococcus 1种；微小杆菌属Exiguobacterium 1种；不动杆菌属Acinetobacter 1种；泛菌属Pantoea 1种；假单孢菌属Pseudomonas 2种；苍白杆菌属Ochrobactrum 1种；微杆菌属Microbacterium 2种。雄性成虫肠道内分离细菌3门8科10属12种，其中梭形杆菌属Lysinibacillus 1种；假杆菌属Fictibacillu 1种；芽孢杆菌属Bacillus 3种；葡萄球菌属Staphylococcus 1种；微小杆菌属Exiguobacterium 1种；肠杆菌属Enterobacter 1种；不动杆菌属Acinetobacter 1种；假单孢菌属Pseudomonas 1种；寡养单胞菌属Stenotrophomonas 1种；微杆菌属Microbacterium 1种。白背飞虱雌雄成虫肠道内细菌共同种类有4种，分别为松鼠葡萄球菌、乙酰微小杆菌、产左聚糖微杆菌和Acinetobacter soli。该研究将为深入研究白背飞虱肠道细菌功能和雌雄虫肠道细菌组成差异及其机理研究提供理论依据。 |
| 英文摘要: |
| In order to clarify the composition of intestinal bacteria of adult Sogatella furcifera（Horváth） and the difference between male and female adults, this study isolated and cultured the composition of intestinal bacteria of adult Sogatella furcifera（Horváth） in paddy field of Yuanjiang County, Yunnan Province by using traditional culture method, and identified the species of bacteria by using morphological and 16S rDNA sequence alignment method. The results showed that 20 species, 12 genera, 8 families, 3 phyla of bacteria were isolated and identified from the intestinal tract of the planthopper. The dominant bacteria Phylum are Firmicides (50%). The dominant bacteria genera in the intestinal tract of female adults are Staphylococcus (25%) and the dominant bacteria species are Staphylococcus squirrel (25%). The dominant bacteria genera in the intestinal tract of male adults are Bacillus (22%) and Microbacter (22%), and the dominant bacteria species are Microbacterium laevaniforman (22%). Among them, 12 species belonging to 8 genera, 8 families and 3 phyla were isolated from the intestinal tract of female adults, including 3 species of Bacillus, 1 species of Staphylococus, 1 species of Acinetobacteris, 1 species of Pantoea, 2 species of Pseudomonas, 1 species of Ochrobactrumis, 2 species of Microbacterium. In the intestinal tract of male adults, 12 species, 10 genera, 8 families and 3 phyla were isolated, including 1 species of Lysinibacillusis, 1 species of Fictibacilluis, 3 species of Bacillusare, 1 species of Staphylococus, 1 species of Exiguobacterium, 1 species of Enterobacter, 1 species of Acinetobacter, 1 species of Pseudomonas, 1 species of Stenotrophomonas, 1 species of Microbacterium. There are four common species of bacteria in the gut of male and female adults of Sogatella furcifera（Horváth）, namely Staphylococcus squirrel, Micrococcus acetylus, Micrococcus dextrogenes and Acinetobacter soli. This study will provide a theoretical basis for the further study of the intestinal bacterial function of Sogatella furcifera（Horváth）, and the difference of intestinal bacterial composition between male and female and its mechanism. |
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